Campus Units

Genetics, Development and Cell Biology

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

2012

Journal or Book Title

Nature

Volume

491

First Page

114

Last Page

118

DOI

10.1038/nature11537

Abstract

The zebrafish (Danio rerio) is increasingly being used to study basic vertebrate biology and human disease using a rich array of in vivo genetic and molecular tools. However, the inability to readily modify the genome in a targeted fashion has been a bottleneck in the field. Here we show that improvements in artificial transcription activator-like effector nucleases (TALENs) provide a powerful new approach for targeted zebrafish genome editing and functional genomic applications1–5. Using the GoldyTALEN modified scaffold and zebrafish delivery system, we show this enhanced TALEN toolkit demonstrates a high efficiency in inducing locus-specific DNA breaks in somatic and germline tissues. At some loci, this efficacy approaches 100%, including biallelic conversion in somatic tissues that mimics phenotypes seen using morpholino (MO)-based targeted gene knockdowns6. With this updated TALEN system, we successfully used single-stranded DNA (ssDNA) oligonucleotides (oligos) to precisely modify sequences at predefined locations in the zebrafish genome through homology-directed repair (HDR), including the introduction of a custom-designed EcoRV site and a modified loxP (mloxP) sequence into somatic tissue in vivo. We further show successful germline transmission of both EcoRV and mloxP engineered chromosomes. This combined approach offers the potential to model genetic variation as well as to generate targeted conditional alleles.

Comments

This is a manuscript of an article published as Bedell, V., Wang, Y., Campbell, J. et al. In vivo genome editing using a high-efficiency TALEN system. Nature 491, 114–118 (2012). doi: 10.1038/nature11537. Posted with permission.

Copyright Owner

Macmillan Publishers Limited

Language

en

File Format

application/pdf

Published Version

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