Document Type
Article
Publication Date
8-2008
Journal or Book Title
Plant Physiology
Volume
147
Issue
4
First Page
1822
Last Page
1829
DOI
10.1104/pp.108.121269
Abstract
The acs1 knockout mutant that has a disruption in the plastidic acetyl-coenzyme A (CoA) synthetase (ACS; At5g36880) gene was used to explore the role of this protein and plastidic acetate metabolism in Arabidopsis (Arabidopsis thaliana). Disruption of the ACS gene decreased ACS activity by 90% and largely blocked the incorporation of exogenous 14C-acetate and 14C-ethanol into fatty acids. Whereas the disruption had no significant effect on the synthesis of bulk seed triacylglycerols, the acs1 plants were smaller and flowered later. This suggests that the pyruvate dehydrogenase bypass provided by the aerobic fermentation pathway that converts pyruvate to acetate and probably on to fatty acids is important to the plants during normal growth. The role of ACS in destroying fermentative intermediates is supported by the increased sensitivity of the acs1mutant to exogenous acetate, ethanol, and acetaldehyde compared to wild-type plants. Whereas these observations suggest that flux through the aerobic fermentation pathway is important, the reason for this flux is unclear. Interestingly, acetate is able to support high rates of plant growth on medium and this growth is blocked in the acs1 mutant.
Copyright Owner
American Society of Plant Biologists
Copyright Date
2008
Language
en
File Format
application/pdf
Recommended Citation
Lin, Ming and Oliver, David J., "The Role of Acetyl-Coenzyme A Synthetase in Arabidopsis" (2008). Genetics, Development and Cell Biology Publications. 4.
https://lib.dr.iastate.edu/gdcb_las_pubs/4
Included in
Agriculture Commons, Biochemistry Commons, Botany Commons, Developmental Biology Commons
Comments
This article is from Plant Physiology 147, no. 4 (August 2008): 1822–1829, doi:10.1104/pp.108.121269.