Campus Units
Agronomy, Genetics, Development and Cell Biology
Document Type
Article
Publication Version
Published Version
Publication Date
2013
Journal or Book Title
Plants
Volume
2
Issue
3
First Page
441
Last Page
454
DOI
10.3390/plants2030441
Abstract
In soybean, genic male sterility can be utilized as a tool to develop hybrid seed. Several male-sterile, female-fertile mutants have been identified in soybean. The male-sterile, female-fertile ms5 mutant was selected after fast neutron irradiation. Male-sterility due to ms5 was associated with the “stay-green” cotyledon color mutation. The cotyledon color trait in soybean is controlled by two loci, D1 and D2. Association between cotyledon color and male-sterility can be instrumental in early phenotypic selection of sterility for hybrid seed production. The use of such selection methods saves time, money, and space, as fewer seeds need to be planted and screened for sterility. The objectives of this study were to compare anther development between male-fertile and male-sterile plants, to investigate the possible linkages among the Ms5, D1 and D2 loci, and to determine if any of the d1 or d2 mutations can be applied in hybrid seed production. The cytological analysis during anther development displayed optically clear, disintegrating microspores and enlarged, engorged pollen in the male-sterile, female-fertile ms5ms5 plants, a common characteristic of male-sterile mutants. The D1 locus was mapped to molecular linkage group (MLG) D1a and was flanked by Satt408 and BARCSOYSSR_01_1622. The ms5 and D2 loci were mapped to MLG B1 with a genetic distance ~12.8 cM between them. These results suggest that use of the d2 mutant in the selection of male-sterile line may attenuate the cost hybrid seed production in soybean.
Rights
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
Copyright Owner
The authors
Copyright Date
2013
Language
en
File Format
application/pdf
Recommended Citation
Ott, Alina; Yang, Yang; Bhattacharyya, Madan; Horner, Harry T.; Palmer, Reid G.; and Sandhu, Devinder, "Molecular Mapping of D1, D2 and ms5 Revealed Linkage between the Cotyledon Color Locus D2 and the Male-Sterile Locus ms5 in Soybean" (2013). Genetics, Development and Cell Biology Publications. 42.
https://lib.dr.iastate.edu/gdcb_las_pubs/42
Included in
Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Breeding and Genetics Commons
Comments
This is article is from Plants 2 (2013); 441, doi: 10.3390/plants2030441. Posted with permission.