Campus Units
Biochemistry, Biophysics and Molecular Biology, Biomedical Sciences, Genetics, Development and Cell Biology, Plant Biology
Document Type
Article
Publication Version
Published Version
Publication Date
2016
Journal or Book Title
Autophagy
Volume
12
Issue
1
First Page
1
Last Page
222
DOI
10.1080/15548627.2015.1100356
Abstract
Many researchers, especially those new to the field, need to determine which criteria are essential for demonstrating autophagy, either for the purposes of their own research, or in the capacity of a manuscript or grant review.1 Acceptable standards are an important issue, particularly considering that each of us may have his/her own opinion regarding the answer. Unfortunately, the answer is in part a “moving target” as the field evolves.2 This can be extremely frustrating for researchers who may think they have met those criteria, only to find out that the reviewers of their papers have different ideas. Conversely, as a reviewer, it is tiresome to raise the same objections repeatedly, wondering why researchers have not fulfilled some of the basic requirements for establishing the occurrence of an autophagic process. In addition, drugs that potentially modulate autophagy are increasingly being used in clinical trials, and screens are being carried out for new drugs that can modulate autophagy for therapeutic purposes. Clearly it is important to determine whether these drugs are truly affecting autophagy, and which step(s) of the process is affected, based on a set of accepted criteria. Accordingly, we describe here a basic set of contemporary guidelines that can be used by researchers to plan and interpret their experiments, by clinicians to evaluate the literature with regard to autophagy-modulating therapies, and by both authors and reviewers to justify or criticize an experimental approach.
Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Recommended Citation
Klionsky, Daniel J.; Abdelmohsen, Kotb; Bassham, Diane C.; Kanthasamy, Anumantha; Macintosh, Gustavo C.; Bai, Hua; and et. al., "Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)" (2016). Genetics, Development and Cell Biology Publications. 52.
https://lib.dr.iastate.edu/gdcb_las_pubs/52
Included in
Cell and Developmental Biology Commons, Genetics and Genomics Commons, Molecular Biology Commons
Comments
This article is from Autophagy 12 (2016): 1, doi: 10.1080/15548627.2015.1100356.