Campus Units
Genetics, Development and Cell Biology
Document Type
Article
Publication Version
Published Version
Publication Date
2014
Journal or Book Title
PloS ONE
Volume
9
Issue
10
DOI
e110194
Abstract
The entire repertoire of intrinsic factors that control the cell fate determination process of specific retinal neurons has yet to be fully identified. Single cell transcriptome profiling experiments of retinal progenitor cells revealed considerable gene expression heterogeneity between individual cells, especially among different classes of transcription factors. In this study, we show that two of those factors, Onecut1 and Onecut2, are expressed during mouse retinal development. Using mice that are deficient for each of these transcription factors, we further demonstrate a significant loss (∼70–80%) of horizontal cells in the absence of either of these proteins, while the other retinal cells appear at normal numbers. Microarray profiling experiments performed on knockout retinas revealed defects in horizontal cell genes as early as E14.5. Additional profiling assays showed an upregulation of several stress response genes in the adult Onecut2 knockout, suggesting that the integrity of the retina is compromised in the absence of normal numbers of horizontal cells. Interestingly, melanopsin, the gene coding for the photopigment found in photosensitive ganglion cells, was observed to be upregulated in Onecut1 deficient retinas, pointing to a possible regulatory role for Onecut1. Taken together, our data show that similar to Onecut1, Onecut2 is also necessary for the formation of normal numbers of horizontal cells in the developing retina.
Rights
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Copyright Owner
Goetz et al.
Copyright Date
2014
Language
en
File Format
application/pdf
Recommended Citation
Goetz, Jillian J.; Martin, Gregory M.; Chowdhury, Rebecca; and Trimarchi, Jeffrey M., "Onecut1 and Onecut2 Play Critical Roles in the Development of the Mouse Retina" (2014). Genetics, Development and Cell Biology Publications. 84.
https://lib.dr.iastate.edu/gdcb_las_pubs/84
Comments
This article is from PloS ONE 9 (2014): e110194, doi: 10.1371/journal.pone.0110194. Posted with permission.