Date

1-4-2016 12:00 AM

Major

Animal Science

Department

Natural Resource Ecology & Management

College

College of Agriculture and Life Sciences

Project Advisor

Dr. Steven Lonergan

Project Advisor's Department

Animal Science

Description

Objective: Residual feed intake (RFI) is a feed efficiency metric that represents the difference between observed and expected feed intake. In a previous experiment, serpinA3 was noticeably more abundant in the serum of efficient low RFI pigs, suggesting potential as a feed efficiency biomarker. Our objective was to determine the effect of selection for residual feed intake on porcine serum SerpinA3 abundance. Materials and Methods: Samples were collected from pigs 35 to 42 days of age. Pigs were fed a traditional or high fiber diet and RFIs were calculated. Pigs from each line and diet (n=7/diet/line, n=28) were selected from a larger group to represent the most extreme RFI values. One-dimensional western blot analysis was performed to determine serpinA3 abundance. Data were analyzed using the mixed procedure of SAS v9.3 with gel as a random effect. Results: RFI line did not significantly affect serpinA3 abundance when using western blot analyisis. In the high fiber diet, serpinA3 was numerically increased in the low RFI line (HRFI=0.84, LRFI=0.98, ±0.09, P= 0.11). SerpinA3 does not appear to be a viable biomarker across diet treatments using western blot analysis, though the high fiber diet showed some promise. This may be caused by western blot technique’s inability to show post-translational protein changes seen in 2D-DIGE. Dietary factors may influence feed efficiency biomarker viability.

File Format

application/pdf

Included in

Meat Science Commons

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Apr 1st, 12:00 AM

Relative SerpinA3 Abundance in Serum Samples as a Possible Predictor of Feed Efficiency in Swine

Objective: Residual feed intake (RFI) is a feed efficiency metric that represents the difference between observed and expected feed intake. In a previous experiment, serpinA3 was noticeably more abundant in the serum of efficient low RFI pigs, suggesting potential as a feed efficiency biomarker. Our objective was to determine the effect of selection for residual feed intake on porcine serum SerpinA3 abundance. Materials and Methods: Samples were collected from pigs 35 to 42 days of age. Pigs were fed a traditional or high fiber diet and RFIs were calculated. Pigs from each line and diet (n=7/diet/line, n=28) were selected from a larger group to represent the most extreme RFI values. One-dimensional western blot analysis was performed to determine serpinA3 abundance. Data were analyzed using the mixed procedure of SAS v9.3 with gel as a random effect. Results: RFI line did not significantly affect serpinA3 abundance when using western blot analyisis. In the high fiber diet, serpinA3 was numerically increased in the low RFI line (HRFI=0.84, LRFI=0.98, ±0.09, P= 0.11). SerpinA3 does not appear to be a viable biomarker across diet treatments using western blot analysis, though the high fiber diet showed some promise. This may be caused by western blot technique’s inability to show post-translational protein changes seen in 2D-DIGE. Dietary factors may influence feed efficiency biomarker viability.