Biochemical characterization of new P450s in rice diterpenoid metabolism
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The Honors project is potentially the most valuable component of an Honors education. Typically Honors students choose to do their projects in their area of study, but some will pick a topic of interest unrelated to their major.
The Honors Program requires that the project be presented at a poster presentation event. Poster presentations are held each semester. Most students present during their senior year, but may do so earlier if their honors project has been completed.
This site presents project descriptions and selected posters for Honors projects completed since the Fall 2015 semester.
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Abstract
The Oryza sativa P450 CYP76M subfamily hydroxylate a range of labdane-related diterpenes (LRD). Previously the CYP76M5-8 enzymes were screened against the range of LRDs found in rice using our metabolic engineering system in Escherichia coli (E. coli) to determine their functions. It was found that, even though these P450s all have very similar amino acid sequences, individual enzymes in this family have different specificities e.g., CYP76M8 is a more promiscuous enzyme, hydroxylating a wider range of LRDs than CYP76M7. This study focuses on screening two more recently discovered P450s in this same family, CYP76M14 & 17. CYP76M14 was found to have a high specificity for a few LRD, whereas CYP76M17 was more promiscuous. This poster will present the data comparing the functions of these six enzymes.