Date

2019 12:00 AM

Major

Genetics

Department

Genetics, Development and Cell Biology

College

Liberal Arts and Sciences

Project Advisor

Leigh Alison Phillips

Description

My research project is for Dr. Michael Kimber regarding schistosomes and the effect of Praziquantel (a drug) on the immune system. This is part of an overall larger project working with his ongoing research of schistosomes. Schistosomiasis affects between 230-440 million people worldwide. Praziquantel is currently the only main drug to treat this disease and it’s largely unknown how it works. Not knowing why it helps may be problematic in the future if the disease develops a resistance to Praziquantel. My project was an independent effort broken into three parts. Initially I was responsible for tissue cultures of frozen J774 macrophages. My responsibility was to pass several generations of these cells in the sterile lab for later testing. Later in the project, I switched to using Bone Marrow Derived Macrophages (BMDM) for the testing of Praziquantel. The second portion of my project was testing for the 5HT2B receptor in these cells. This receptor must be present for my data collection project to be carried over as useful information to humans being treated with Praziquantel. In the final phase, after proving this receptor is present in the BMDM cells, I treated these cells with Praziquantel and tested for any changes in the immune response for these cells. My results showed that overall there does not appear to be a significant change in the immune response of BMDM cells treated with either S-PZQ or R-PZQ, but another replicate of my project would need to be performed to determine any significance of data. This information could possibly help drug developers in their efforts to design an additional drug to treat Schistosomiasis.

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Jan 1st, 12:00 AM

Interaction of Praziquantel with Host Innate Immune Cells

My research project is for Dr. Michael Kimber regarding schistosomes and the effect of Praziquantel (a drug) on the immune system. This is part of an overall larger project working with his ongoing research of schistosomes. Schistosomiasis affects between 230-440 million people worldwide. Praziquantel is currently the only main drug to treat this disease and it’s largely unknown how it works. Not knowing why it helps may be problematic in the future if the disease develops a resistance to Praziquantel. My project was an independent effort broken into three parts. Initially I was responsible for tissue cultures of frozen J774 macrophages. My responsibility was to pass several generations of these cells in the sterile lab for later testing. Later in the project, I switched to using Bone Marrow Derived Macrophages (BMDM) for the testing of Praziquantel. The second portion of my project was testing for the 5HT2B receptor in these cells. This receptor must be present for my data collection project to be carried over as useful information to humans being treated with Praziquantel. In the final phase, after proving this receptor is present in the BMDM cells, I treated these cells with Praziquantel and tested for any changes in the immune response for these cells. My results showed that overall there does not appear to be a significant change in the immune response of BMDM cells treated with either S-PZQ or R-PZQ, but another replicate of my project would need to be performed to determine any significance of data. This information could possibly help drug developers in their efforts to design an additional drug to treat Schistosomiasis.