Date

2019 12:00 AM

Major

Entomology

Department

Entomology

College

Liberal Arts and Sciences

Project Advisor

Ryan Smith

Description

This semester, we wanted to further investigate the functions of Eiger and Wengen in mosquito immunity. Last semester, we found that following the knockdown of the Eiger and Wengen genes, there was a significant decrease in oocyst survival rates in the midgut eight days post-infection. We wanted to confirm that this pattern was caused by a reduced expression of the respective genes, so we measured levels of expression of both genes following silencing. We found significant reduction of expression of both Eiger and Wengen in the mosquitos that were treated with the respective RNAi knockdown. This confirmed that our RNAi was effective in reducing gene expression, and that our previous results could be attributed to the success of the knockdown. After this confirmation experiment was completed, we evaluated the expression profiles of both Eiger and Wengen in three different mosquito tissue sites (fat body, midgut, and hemolymph) under different conditions (non-blood fed, blood fed, and blood fed with Plasmodium berghei). We used qrtPCR to determine levels of Eiger and Wengen expression in under each combination of conditions, but we could find no significant pattern in our results.

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Jan 1st, 12:00 AM

Investigation of the msoquito gene Eiger and its receptor Wengen

This semester, we wanted to further investigate the functions of Eiger and Wengen in mosquito immunity. Last semester, we found that following the knockdown of the Eiger and Wengen genes, there was a significant decrease in oocyst survival rates in the midgut eight days post-infection. We wanted to confirm that this pattern was caused by a reduced expression of the respective genes, so we measured levels of expression of both genes following silencing. We found significant reduction of expression of both Eiger and Wengen in the mosquitos that were treated with the respective RNAi knockdown. This confirmed that our RNAi was effective in reducing gene expression, and that our previous results could be attributed to the success of the knockdown. After this confirmation experiment was completed, we evaluated the expression profiles of both Eiger and Wengen in three different mosquito tissue sites (fat body, midgut, and hemolymph) under different conditions (non-blood fed, blood fed, and blood fed with Plasmodium berghei). We used qrtPCR to determine levels of Eiger and Wengen expression in under each combination of conditions, but we could find no significant pattern in our results.