Document Type

Article

Publication Version

Published Version

Publication Date

11-2014

Journal or Book Title

Cryoletter

Volume

35

Issue

6

First Page

507

Last Page

515

Abstract

BACKGROUND: Methods are needed for the conservation of clonally maintained trees of Populus and Salix. In this work, Populus trichocarpa and Salix genetic resources were cryopreserved using dormant scions as the source explant. OBJECTIVE: We quantified the recovery of cryopreserved materials that originated from diverse field environments by using either direct sprouting or grafting. MATERIALS AND METHODS: Scions (either at their original moisture content of 48 to 60% or dried to 30%) were slowly cooled to -35°C, transferred to the vapor phase of liquid nitrogen (LNV, -160°C), and warmed before determining survival. RESULTS: Dormant buds from P. trichocarpa clones from Westport and Boardman, OR had regrowth levels between 42 and 100%. Direct rooting of cryopreserved P. trichocarpa was also possible. Ten of 11 cryopreserved Salix accessions, representing 10 different species, exhibited at least 40% bud growth and rooting after 6 weeks when a bottom-heated rooting system was implemented. CONCLUSION: We demonstrate that dormant buds of P. trichocarpa and Salixaccessions can be cryopreserved and successfully regenerated without the use of tissue culture.

Comments

This article is from Cryoletters 35 (2014): 507–515.

Rights

Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.

Language

en

File Format

application/pdf

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