One of the basic tenets of plant biosecurity is that the presence, actual or predicted distribution, intensity, and economic impact of any yield-reducing factor(s) must be known. The development of a real-time, GIS-based (geographic information system) reporting system for new and emerging agricultural pathogens and pests is extremely relevant in the era of agricultural bioterrorism. The goal is to establish a real-time, GIS database network to report, monitor, map (temporally and spatially), and predict the spread of new and emerging plant diseases and pests. This database network can also be used to geospatially and temporally monitor endemic pathogens/pests. Diagnostic records from the Regional Diagnostic Centers coupled with remote sensing, GIS, GPS, atmospheric transport models, and weather-based GIS risk prediction models, offers an integrated system of technologies to help ensure the production of a safe and affordable US food supply.