Campus Units

Industrial and Manufacturing Systems Engineering

Document Type

Article

Publication Version

Published Version

Publication Date

8-22-2018

Journal or Book Title

Journal of Applied Polymer Science

Volume

135

First Page

46692

DOI

10.1002/app.46692

Abstract

This study presents and evaluates an approach for fabricating poly(lactic acid) (PLA)/chitosan (CS)/tricalcium phosphate (TCP) electrospun scaffolds for guided bone regeneration, a dental procedure that uses membranes to direct and delineate regions of osteogenesis. Biomaterials were pre‐processed using cryomilling, a solid‐state grinding technique that facilitates the generation of powdered biocomposites conducive to electrospinning. X‐ray diffraction (XRD) confirmed the generation of cryomilled blends consisting of PLA, CS, and TCP. Results from the differential scanning calorimetry showed an upward shift in glass transition temperature and an increase in crystallinity with the inclusion of TCP reinforcing the observations from XRD. Murine macrophages were used to confirm the biocompatibility of the cryomilled powders and was evaluated using CellTiter‐Blue (CTB) cell viability assay and brightfield microscopy. Scanning electron microscopy was used to examine the morphology of the fibers produced via electrospinning, while Raman spectroscopy confirmed material homogeneity. In vitro studies with MG‐63 cells validated the capacity of composite scaffolds to encourage proliferation, while Coherent anti‐Stokes Raman scattering and fluorescence microscopies provided visual evidence of cell proliferation. CTB assay revealed that cells maintain viability and metabolic activity at 3 and 7 days after seeding, demonstrating the potential of the biocomposite membranes.

Comments

This article is published as Ramesh, Srikanthan, Lisa Lungaro, Dimitrios Tsikritsis, Eric Weflen, Iris V. Rivero, and Alistair PD Elfick. "Fabrication and evaluation of poly (lactic acid), chitosan, and tricalcium phosphate biocomposites for guided bone regeneration." Journal of Applied Polymer Science 135 (2018): 46692. DOI: 10.1002/app.46692. Posted with permission.

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

Copyright Owner

The Authors

Language

en

File Format

application/pdf

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