On Jan. 31, 1951, a 4-year-old shorthorn cow was admitted to Stange Memorial Clinic, and on Mar. 31, 1951, a 5- year-old Brown Swiss bull was also admitted. Both animals were in poor general condition-showing symptoms of watery diarrhea, atonic rumen and poor appetite. Both patients were treated symptomatically for several days with little improvement. It was decided to subject both animals to a test for Johne's disease. The test was conducted as follows: both animals received 10 cc. of avian tuberculin intravenously and their temperatures were checked hourly.
Freeman, E. M.
"A case of Johne's Disease,"
Iowa State University Veterinarian: Vol. 13
, Article 16.
Available at: https://lib.dr.iastate.edu/iowastate_veterinarian/vol13/iss3/16