Project ID



This multi-scale, multi-year study was conducted to begin answering questions related to the fate and transport of nutrients and bacteria from land receiving manure application. Experiments were conducted on tile water drainage at an ISU Research Farm in northeast Iowa.

Key Question

What can we learn from comparing cropping systems and fertilization about N export, bacterial survival and bacterial transport mechanisms that will help us better manage cropping systems?


Over three years, fecal indicator bacteria present in tile drainage water from swine-amended cropland were extremely low, except in high precipitation events. Fall-injected swine manure had low concentrations of pathogen indicator and resistant organisms, but higher N export in tile drain water. Indicator isolates collected from manure and tested for resistance to multiple antibiotics exhibited higher resistance than those pulled from the soil and water isolates.

Principal Investigator(s)

Michelle Soupir


Matt Helmers, Michael Thompson, Laura Jarboe, Antonio Mallarino, Ramesh Kanwar

Year of Grant Completion