Document Type
Conference Proceeding
Conference
Frontiers in Biological Detection: From Nanosensors to Systems IV
Publication Version
Published Version
Publication Date
2-2012
Journal or Book Title
Proceedings of SPIE - The International Society for Optical Engineering
Volume
8212
First Page
1
Last Page
6
DOI
10.1117/12.905679
Conference Title
Frontiers in Biological Detection: From Nanosensors to Systems IV
Conference Date
January 21, 2012
City
San Francisco, CA
Abstract
Analysis of the intrinsic scatter and fluorescence profiles of marine algae can be used for general classification of organisms based on cell size and fluorescence properties. We describe the design and fabrication of a Microflow Cytometer on a chip for characterization of phytoplankton. The Microflow Cytometer measured distinct side-scatter and fluorescence properties of Synechococcus sp., Nitzschia d., and Thalassiosira p. Measurements were confirmed using the benchtop Accuri C6 flow cytometer. The Microflow Cytometer proved sensitive enough to detect and characterize picoplankton with diameter approximately 1 mm and larger phytoplankton of up to 80 mm in length. The wide range in size discrimination coupled with detection of intrinsic fluorescent pigments suggests that this Microflow Cytometer will be able to distinguish different populations of phytoplankton on unmanned underwater vehicles. Reversing the orientation of the grooves in the channel walls returns the sample stream to its original unsheathed position allowing separation of the sample stream from the sheath streams and the recycling of the sheath fluid.
Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Recommended Citation
Golden, Joel P.; Hashemi, Nicole N.; Erickson, Jeffrey S.; and Ligler, Frances S., "A microflow cytometer for optical analysis of phytoplankton" (2012). Mechanical Engineering Conference Presentations, Papers, and Proceedings. 156.
https://lib.dr.iastate.edu/me_conf/156
Included in
Acoustics, Dynamics, and Controls Commons, Marine Biology Commons, Other Oceanography and Atmospheric Sciences and Meteorology Commons
Comments
This is a conference proceeding from Proceeding of SPIE 8212 (2012): 1, doi:10.1117/12.905679. Posted with permission.