Document Type

Conference Proceeding

Conference

Frontiers in Biological Detection: From Nanosensors to Systems IV

Publication Version

Published Version

Publication Date

2-2012

Journal or Book Title

Proceedings of SPIE - The International Society for Optical Engineering

Volume

8212

First Page

1

Last Page

6

DOI

10.1117/12.905679

Conference Title

Frontiers in Biological Detection: From Nanosensors to Systems IV

Conference Date

January 21, 2012

City

San Francisco, CA

Abstract

Analysis of the intrinsic scatter and fluorescence profiles of marine algae can be used for general classification of organisms based on cell size and fluorescence properties. We describe the design and fabrication of a Microflow Cytometer on a chip for characterization of phytoplankton. The Microflow Cytometer measured distinct side-scatter and fluorescence properties of Synechococcus sp., Nitzschia d., and Thalassiosira p. Measurements were confirmed using the benchtop Accuri C6 flow cytometer. The Microflow Cytometer proved sensitive enough to detect and characterize picoplankton with diameter approximately 1 mm and larger phytoplankton of up to 80 mm in length. The wide range in size discrimination coupled with detection of intrinsic fluorescent pigments suggests that this Microflow Cytometer will be able to distinguish different populations of phytoplankton on unmanned underwater vehicles. Reversing the orientation of the grooves in the channel walls returns the sample stream to its original unsheathed position allowing separation of the sample stream from the sheath streams and the recycling of the sheath fluid.

Comments

This is a conference proceeding from Proceeding of SPIE 8212 (2012): 1, doi:10.1117/12.905679. Posted with permission.

Rights

Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.

Language

en

File Format

application/pdf

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