Campus Units

Mechanical Engineering, Agronomy, Electrical and Computer Engineering, Plant Sciences Institute

Document Type

Article

Publication Version

Published Version

Publication Date

1-23-2020

Journal or Book Title

Plant Methods

Volume

16

First Page

5

DOI

10.1186/s13007-019-0550-5

Abstract

Background Root system architecture (RSA) traits are of interest for breeding selection; however, measurement of these traits is difficult, resource intensive, and results in large variability. The advent of computer vision and machine learning (ML) enabled trait extraction and measurement has renewed interest in utilizing RSA traits for genetic enhancement to develop more robust and resilient crop cultivars. We developed a mobile, low-cost, and high-resolution root phenotyping system composed of an imaging platform with computer vision and ML based segmentation approach to establish a seamless end-to-end pipeline - from obtaining large quantities of root samples through image based trait processing and analysis.

Results This high throughput phenotyping system, which has the capacity to handle hundreds to thousands of plants, integrates time series image capture coupled with automated image processing that uses optical character recognition (OCR) to identify seedlings via barcode, followed by robust segmentation integrating convolutional auto-encoder (CAE) method prior to feature extraction. The pipeline includes an updated and customized version of the Automatic Root Imaging Analysis (ARIA) root phenotyping software. Using this system, we studied diverse soybean accessions from a wide geographical distribution and report genetic variability for RSA traits, including root shape, length, number, mass, and angle.

Conclusions This system provides a high-throughput, cost effective, non-destructive methodology that delivers biologically relevant time-series data on root growth and development for phenomics, genomics, and plant breeding applications. This phenotyping platform is designed to quantify root traits and rank genotypes in a common environment thereby serving as a selection tool for use in plant breeding. Root phenotyping platforms and image based phenotyping are essential to mirror the current focus on shoot phenotyping in breeding efforts.

Comments

This article is published as Falk, Kevin G., Talukder Z. Jubery, Seyed V. Mirnezami, Kyle A. Parmley, Soumik Sarkar, Arti Singh, Baskar Ganapathysubramanian, and Asheesh K. Singh. "Computer vision and machine learning enabled soybean root phenotyping pipeline." Plant Methods 16 (2020): 5. DOI: 10.1186/s13007-019-0550-5. Posted with permission.

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Copyright Owner

The Author(s)

Language

en

File Format

application/pdf

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