Campus Units
Mechanical Engineering, Biomedical Sciences, Ames Laboratory, Bioeconomy Institute (BEI), Genetics, Development and Cell Biology, Neuroscience
Document Type
Article
Publication Version
Accepted Manuscript
Publication Date
2-2019
Journal or Book Title
Macromolecular Bioscience
Volume
19
Issue
2
First Page
1800236
DOI
10.1002/mabi.201800236
Abstract
Biomaterials are essential for the development of innovative biomedical and therapeutic applications. Biomaterials‐based scaffolds can influence directed cell differentiation to improve cell‐based strategies. Using a novel microfluidics approach, poly (ε‐caprolactone) (PCL), is used to fabricate microfibers with varying diameters (3–40 µm) and topographies (straight and wavy). Multipotent adult rat hippocampal stem/progenitor cells (AHPCs) are cultured on 3D aligned PCL microfibrous scaffolds to investigate their ability to differentiate into neurons, astrocytes, and oligodendrocytes. The results indicate that the PCL microfibers significantly enhance proliferation of the AHPCs compared to control, 2D planar substrates. While the AHPCs maintained their multipotent differentiation capacity when cultured on the PCL scaffolds, there is a significant and dramatic increase in immunolabeling for astrocyte and oligodendrocyte differentiation when compared with growth on planar surfaces. Our results show a 3.5‐fold increase in proliferation and 23.4‐fold increase in astrocyte differentiation for cells on microfibers. Transplantation of neural stem/progenitor cells within a PCL microfiber scaffold may provide important biological and topographic cues that facilitate the survival, selective differentiation, and integration of transplanted cells to improve therapeutic strategies.
Copyright Owner
WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
Copyright Date
2018
Language
en
File Format
application/pdf
Recommended Citation
Patel, Bhavika B.; Sharifi, Farrokh; Stroud, Daniel P.; Montazami, Reza; Hashemi, Nicole N.; and Sakaguchi, Donald S., "3D Microfibrous Scaffolds Selectively Promotes Proliferation and Glial Differentiation of Adult Neural Stem Cells: A Platform to Tune Cellular Behavior in Neural Tissue Engineering" (2019). Mechanical Engineering Publications. 409.
https://lib.dr.iastate.edu/me_pubs/409
Included in
Biology and Biomimetic Materials Commons, Biomedical Engineering and Bioengineering Commons
Comments
This is the peer-reviewed version of the following article: Patel, Bhavika B., Farrokh Sharifi, Daniel P. Stroud, Reza Montazami, Nicole N. Hashemi, and Donald S. Sakaguchi. "3D microfibrous scaffolds selectively promotes proliferation and glial differentiation of adult neural stem cells: a platform to tune cellular behavior in neural tissue engineering." Macromolecular Bioscience 19, no. 2 (2019): 1800236, which has been published in final form at DOI: 10.1002/mabi.201800236. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. Posted with permission.