Campus Units

Mechanical Engineering, Biomedical Sciences, Ames Laboratory, Bioeconomy Institute (BEI), Genetics, Development and Cell Biology, Neuroscience

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

2-2019

Journal or Book Title

Macromolecular Bioscience

Volume

19

Issue

2

First Page

1800236

DOI

10.1002/mabi.201800236

Abstract

Biomaterials are essential for the development of innovative biomedical and therapeutic applications. Biomaterials‐based scaffolds can influence directed cell differentiation to improve cell‐based strategies. Using a novel microfluidics approach, poly (ε‐caprolactone) (PCL), is used to fabricate microfibers with varying diameters (3–40 µm) and topographies (straight and wavy). Multipotent adult rat hippocampal stem/progenitor cells (AHPCs) are cultured on 3D aligned PCL microfibrous scaffolds to investigate their ability to differentiate into neurons, astrocytes, and oligodendrocytes. The results indicate that the PCL microfibers significantly enhance proliferation of the AHPCs compared to control, 2D planar substrates. While the AHPCs maintained their multipotent differentiation capacity when cultured on the PCL scaffolds, there is a significant and dramatic increase in immunolabeling for astrocyte and oligodendrocyte differentiation when compared with growth on planar surfaces. Our results show a 3.5‐fold increase in proliferation and 23.4‐fold increase in astrocyte differentiation for cells on microfibers. Transplantation of neural stem/progenitor cells within a PCL microfiber scaffold may provide important biological and topographic cues that facilitate the survival, selective differentiation, and integration of transplanted cells to improve therapeutic strategies.

Comments

This is the peer-reviewed version of the following article: Patel, Bhavika B., Farrokh Sharifi, Daniel P. Stroud, Reza Montazami, Nicole N. Hashemi, and Donald S. Sakaguchi. "3D microfibrous scaffolds selectively promotes proliferation and glial differentiation of adult neural stem cells: a platform to tune cellular behavior in neural tissue engineering." Macromolecular Bioscience 19, no. 2 (2019): 1800236, which has been published in final form at DOI: 10.1002/mabi.201800236. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. Posted with permission.

Copyright Owner

WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim

Language

en

File Format

application/pdf

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