Campus Units

Mechanical Engineering, Biomedical Sciences, Ames Laboratory, Bioeconomy Institute (BEI)

Document Type

Article

Publication Version

Submitted Manuscript

Publication Date

5-28-2021

Journal or Book Title

ACS Macro Letters

Volume

10

First Page

732

Last Page

736

DOI

10.1021/acsmacrolett.1c00159

Abstract

At present, the blood–brain barrier (BBB) poses a challenge for treating a wide range of central nervous system disorders; reliable BBB models are still needed to understand and manipulate the transfer of molecules into the brain, thereby improving the efficiency of treatments. In this study, hollow, cell-laden microfibers are fabricated and investigated as a starting point for generating BBB models. The genetic effects of the manufacturing process are analyzed to understand the implications of encapsulating cells in this manner. These fibers are created using different manufacturing parameters to understand the effects on wall thickness and overall diameter. Then, dopaminergic rat cells are encapsulated into hollow fibers, which maintained at least 60% live cells throughout the three-day observation period. Lastly, genetic changes tyrosine hydroxylase (TH) and tubulin beta 3 class III (TUBB-3) are investigated to elucidate the effects on cell health and behavior; while the TH levels in encapsulated cells were similar to control cells, showing similar levels of TH synthesis, TUBB-3 was downregulated, indicating lower amounts of cellular neurogenesis.

Comments

This document is the unedited Author’s version of a Submitted Work that was subsequently accepted for publication in ACS Macro Letters, copyright © American Chemical Society after peer review. To access the final edited and published work see DOI: 10.1021/acsmacrolett.1c00159. Posted with permission.

Copyright Owner

American Chemical Society

Language

en

File Format

application/pdf

Published Version

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