Campus Units
Materials Science and Engineering, Chemical and Biological Engineering, Ames Laboratory
Document Type
Article
Publication Version
Accepted Manuscript
Publication Date
12-7-2018
Journal or Book Title
ACS Applied Bio Materials
DOI
10.1021/acsabm.8b00562
Abstract
In tissue engineering scaffolds, macrophages play a critical role in determining the host response to implanted biomaterials. Macrophage phenotype is dynamic throughout the host response, and a balance of phenotypes is essential for timely progression from injury to proper wound healing. Therefore, it is important to predict how materials will modulate the response of macrophages. In this study, we investigated the effect of methacrylated gellan gum hydrogels on macrophage phenotype and proliferation with the ultimate goal of improving rational design of biomedical implants. Naïve, along with classically and alternatively activated RAW 264.7 macrophages were seeded on methacrylated gellan gum hydrogels that were fabricated with different thiol-ene ratios and crosslinking mechanisms. Live/dead assays showed that all hydrogels supported cell attachment and proliferation. Stiffer substrates enhanced anti-inflammatory production of nitrites from both naïve and classically activated macrophages compared to the softer substrates. Moreover, arginine and CD206 expression – markers for alternatively activated macrophages – were inhibited by higher thiol content. Introducing ionic crosslinks using calcium did not influence the proliferation or polarization for any of the three macrophage phenotypes. Our results suggest that the macrophage phenotype shift from M1 to M2 is controlled by the different crosslinking mechanisms, physical properties, and the chemistry of methacrylated gellan gum hydrogels.
Copyright Owner
American Chemical Society
Copyright Date
2018
Language
en
File Format
application/pdf
Recommended Citation
Li, Zhuqing and Bratlie, Kaitlin M., "How crosslinking Mechanisms of Methacrylated Gellan Gum Hydrogels Alter Macrophage Phenotype" (2018). Materials Science and Engineering Publications. 318.
https://lib.dr.iastate.edu/mse_pubs/318
Included in
Biochemical and Biomolecular Engineering Commons, Biology and Biomimetic Materials Commons, Biomaterials Commons
Comments
This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Applied Bio Materials, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see DOI: 10.1021/acsabm.8b00562. Posted with permission.