Campus Units

Materials Science and Engineering, Chemical and Biological Engineering, Ames Laboratory

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

1-14-2019

Journal or Book Title

Biomaterials Science

DOI

10.1039/C8BM01535J

Abstract

We fabricated photocrosslinked, environmentally responsive alginate hydrogels for tissue engineering applications. Methacrylated alginate (ALGMA) hydrogels were prepared across a variety and combination of ionic and covalent (chain growth, step growth, and mixed mode) crosslinking strategies to obtain a range of compressive moduli from 9.3 ± 0.2 kPa for the softest ionically crosslinked hydrogels to 22.6 ± 0.3 kPa for the dually crosslinked ionic mixed mode gels. The swelling behavior of the alginate hydrogels was significantly higher under basic pH conditions. Stiffer gels consistently swelled to a lesser degree compared to softer gels for all conditions. These hydrogels were stable – retaining >80% of their original mass for three weeks – when incubated in a basic solution of diluted sodium hydroxide, which mimicked accelerated degradation conditions. Encapsulated NIH/3T3 fibroblasts remained viable and proliferated significantly more in stiffer hydrogel substrates compared to softer gels. Additionally, the collagen secreted by encapsulated fibroblasts was quantifiably compared using second harmonic generation (SHG) imaging. Fibroblasts encapsulated in the softer hydrogels secreted significantly less collagen than the stiffer gels. The collagen in these softer gels was also more aligned than the stiffer gels. The ability to tune collagen organization using hydrogels has potential applications ranging from corneal wound healing where organized collagen is desired to epithelial wound scaffolds where a random organization is preferable.

Comments

This is a manuscript of an article published as Boddapalli, Anuraag, and Kaitlin M. Bratlie. "Second harmonic generation microscopy of collagen organization in tunable, environmentally responsive alginate hydrogels." Biomaterials Science (2019). doi: 10.1039/C8BM01535J. Posted with permission.

Copyright Owner

The Royal Society of Chemistry

Language

en

File Format

application/pdf

Available for download on Tuesday, January 14, 2020

Published Version

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