Campus Units
Natural Resource Ecology and Management
Document Type
Article
Publication Version
Submitted Manuscript
Publication Date
1-11-2021
Journal or Book Title
Research Square
DOI
10.21203/rs.3.rs-140573/v1
Abstract
Detailed information on species delineation and population genetic structure is a prerequisite for designing effective restoration and conservation strategies for imperiled organisms. Phylogenomic and population genomic analyses based on genome-wide ddRAD-Seq data has identified three allopatric lineages in the North American freshwater mussel genus Cyprogenia. Cyprogenia stegaria is restricted to the Eastern Highlands and displays little genetic structuring within this region. However, two allopatric clusters of C. aberti in the Ozark and Ouachita highlands exhibit substantial levels (mean FST = 0.120) of genetic differentiation and each warrants recognition as a distinct evolutionary lineage. Lineages of Cyprogenia in the Ouachita and Ozark highlands are further subdivided reflecting structuring at the level of river systems. Species tree inference and species delimitation in a Bayesian framework using SNP data supported results from phylogenetic analyses, which favors recognizing three species of Cyprogenia over the currently recognized two species.
A comparison of SNPs generated from both destructively and non-destructively collected samples revealed no significant difference in the SNP error rate, quality and amount of ddRAD sequence reads, indicating that nondestructive or trace samples can be effectively utilized to generate SNP data for organisms for which destructive sampling is not permitted.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Copyright Owner
The Authors
Copyright Date
2021
Language
en
File Format
application/pdf
Recommended Citation
Roe, Kevin and Kim, Kyung Seok, "Genome-Wide SNPs Redefines Species Boundaries and Conservation Units in the Freshwater Mussel Genus Cyprogenia of North America" (2021). Natural Resource Ecology and Management Publications. 368.
https://lib.dr.iastate.edu/nrem_pubs/368
Included in
Ecology and Evolutionary Biology Commons, Genetics and Genomics Commons, Natural Resources and Conservation Commons, Natural Resources Management and Policy Commons
Comments
This preprint is made available through Research Square at doi: 10.21203/rs.3.rs-140573/v1.