Campus Units
Plant Pathology and Microbiology
Document Type
Article
Publication Version
Published Version
Publication Date
2-2011
Journal or Book Title
The Plant Journal
Volume
65
Issue
3
First Page
430
Last Page
440
DOI
10.1111/j.1365-313X.2010.04433.x
Abstract
Plant-parasitic cyst nematodes secrete CLAVATA3 (CLV3)/ESR (CLE)-like effector proteins. These proteins have been shown to act as ligand mimics of plant CLE peptides and are required for successful nematode infection; however, the receptors for nematode CLE-like peptides have not been identified. Here we demonstrate that CLV2 and CORYNE (CRN), members of the receptor kinase family, are required for nematode CLE signaling. Exogenous peptide assays and overexpression of nematode CLEs in Arabidopsis demonstrated that CLV2 and CRN are required for perception of nematode CLEs. In addition, promoter–reporter assays showed that both receptors are expressed in nematode-induced syncytia. Lastly, infection assays with receptor mutants revealed a decrease in both nematode infection and syncytium size. Taken together, our results indicate that perception of nematode CLEs by CLV2 and CRN is not only required for successful nematode infection but is also involved in the formation and/or maintenance of nematode-induced syncytia.
Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Recommended Citation
Replogle, Amy; Wang, Jianying; Bleckmann, Andrea; Hussey, Richard S.; Baum, Thomas J.; Sawa, Shinichiro; Davis, Eric L.; Wang, Xianhong; Simon, Rudiger; and Mitchum, Melissa G., "Nematode CLE signaling in Arabidopsis requires CLAVATA2 and CORYNE" (2011). Plant Pathology and Microbiology Publications. 152.
https://lib.dr.iastate.edu/plantpath_pubs/152
Included in
Agricultural Science Commons, Agriculture Commons, Entomology Commons, Plant Pathology Commons
Comments
This article is published as Replogle, Amy, Jianying Wang, Andrea Bleckmann, Richard S. Hussey, Thomas J. Baum, Shinichiro Sawa, Eric L. Davis, Xiaohong Wang, Rüdiger Simon, and Melissa G. Mitchum. "Nematode CLE signaling in Arabidopsis requires CLAVATA2 and CORYNE." The Plant Journal 65, no. 3 (2011): 430-440, doi: 10.1111/j.1365-313X.2010.04433.x.