Campus Units
Plant Pathology and Microbiology
Document Type
Article
Publication Version
Published Version
Publication Date
1-1999
Journal or Book Title
Molecular Plant-Microbe Interactions
Volume
12
Issue
1
First Page
64
Last Page
67
DOI
10.1094/MPMI.1999.12.1.64
Abstract
Polyclonal sera specific to β-1,4-endoglucanases (cellulases) synthesized in the subventral esophageal gland cells of the soybean cyst nematode, Heterodera glycines, were used to provide the first identification of a nematode esophageal gland protein that is secreted into host plant tissue. Sera generated to proteins encoded by Hg-eng-1 and Hg-eng-2(endoglucanases) did not cross-react with soybean root proteins on Western blots (immunoblots) or in immunofluorescence microscopy of noninoculated (control) soybean root sections. In cross sections of soybean roots at 24 h after inoculation of roots with second-stage juveniles of H. glycines, HG-ENG-1 was localized within the nematode's subventral gland cells and was not detected in root tissue. HG-ENG-2 was localized within the subventral gland cells and was secreted from the juvenile's cortical tissue at 24 h after inoculation of roots with second-stage juveniles of H. glycines. HG-ENG-2 was localized along the juvenile's migratory path through the root cortex.
Copyright Owner
The American Phytopathological Society
Copyright Date
1999
Language
en
File Format
application/pdf
Recommended Citation
Wang, Xiaohong; Meyers, Diane; Yan, Yitang; Baum, Thomas; Smant, Geert; Hussey, Richard; and Davis, Eric, "In Planta Localization of a β-1,4-Endoglucanase Secreted by Heterodera glycines" (1999). Plant Pathology and Microbiology Publications. 155.
https://lib.dr.iastate.edu/plantpath_pubs/155
Included in
Agricultural Science Commons, Agriculture Commons, Plant Breeding and Genetics Commons, Plant Pathology Commons
Comments
This article is published as Wang, Xiaohong, Diane Meyers, Yitang Yan, Thomas Baum, Geert Smant, Richard Hussey, and Eric Davis. "In planta localization of a β-1, 4-endoglucanase secreted by Heterodera glycines." Molecular Plant-Microbe Interactions 12, no. 1 (1999): 64-67, doi: 10.1094/MPMI.1999.12.1.64. Posted with permission.