Plant Pathology and Microbiology
Journal or Book Title
Molecular Plant-Microbe Interactions
Polyclonal sera specific to β-1,4-endoglucanases (cellulases) synthesized in the subventral esophageal gland cells of the soybean cyst nematode, Heterodera glycines, were used to provide the first identification of a nematode esophageal gland protein that is secreted into host plant tissue. Sera generated to proteins encoded by Hg-eng-1 and Hg-eng-2(endoglucanases) did not cross-react with soybean root proteins on Western blots (immunoblots) or in immunofluorescence microscopy of noninoculated (control) soybean root sections. In cross sections of soybean roots at 24 h after inoculation of roots with second-stage juveniles of H. glycines, HG-ENG-1 was localized within the nematode's subventral gland cells and was not detected in root tissue. HG-ENG-2 was localized within the subventral gland cells and was secreted from the juvenile's cortical tissue at 24 h after inoculation of roots with second-stage juveniles of H. glycines. HG-ENG-2 was localized along the juvenile's migratory path through the root cortex.
The American Phytopathological Society
Wang, Xiaohong; Meyers, Diane; Yan, Yitang; Baum, Thomas; Smant, Geert; Hussey, Richard; and Davis, Eric, "In Planta Localization of a β-1,4-Endoglucanase Secreted by Heterodera glycines" (1999). Plant Pathology and Microbiology Publications. 155.