Campus Units

Plant Pathology and Microbiology

Document Type

Article

Publication Version

Published Version

Publication Date

10-2001

Journal or Book Title

Molecular Plant-Microbe Interactions

Volume

14

Issue

10

First Page

1247

Last Page

1254

DOI

10.1094/MPMI.2001.14.10.1247

Abstract

Cloning parasitism genes encoding secretory proteins expressed in the esophageal gland cells is the key to understanding the molecular basis of nematode parasitism of plants. Suppression subtractive hybridization (SSH) with the microaspirated contents from Heterodera glycines esophageal gland cells and intestinal region was used to isolate genes expressed preferentially in the gland cells of parasitic stages. Twenty-three unique cDNA sequences from a SSH cDNA library were identified and hybridized to the genomic DNA of H. glycines in Southern blots. Full-length cDNAs of 21 clones were obtained by screening a gland-cell long-distance polymerase chain reaction cDNA library. Deduced proteins of 10 clones were preceded by a signal peptide for secretion, and PSORT II computer analysis predicted eight proteins as extracellular, one as nuclear, and one as plasmalemma localized. In situ hybridization showed that four of the predicted extracellular clones were expressed specifically in the dorsal gland cell, one in the subventral gland cells, and three in the intestine in H. glycines. The predicted nuclear clone and the plasmalemma-localized clone were expressed in the subventral gland cells and the dorsal gland cell, respectively. SSH is an efficient method for cloning putative parasitism genes encoding esophageal gland cell secretory proteins that may have a role in H. glycines parasitism of soybean.

Comments

This article is published as Gao, Bingli, R. Allen, Tom Maier, Eric L. Davis, Thomas J. Baum, and Richard S. Hussey. "Identification of putative parasitism genes expressed in the esophageal gland cells of the soybean cyst nematode Heterodera glycines." Molecular Plant-Microbe Interactions 14, no. 10 (2001): 1247-1254, doi: 10.1094/MPMI.2001.14.10.1247. Posted with permission.

Copyright Owner

The American Phytopathological Society

Language

en

File Format

application/pdf

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