Campus Units
Plant Pathology and Microbiology
Document Type
Article
Publication Version
Published Version
Publication Date
10-2001
Journal or Book Title
Molecular Plant-Microbe Interactions
Volume
14
Issue
10
First Page
1247
Last Page
1254
DOI
10.1094/MPMI.2001.14.10.1247
Abstract
Cloning parasitism genes encoding secretory proteins expressed in the esophageal gland cells is the key to understanding the molecular basis of nematode parasitism of plants. Suppression subtractive hybridization (SSH) with the microaspirated contents from Heterodera glycines esophageal gland cells and intestinal region was used to isolate genes expressed preferentially in the gland cells of parasitic stages. Twenty-three unique cDNA sequences from a SSH cDNA library were identified and hybridized to the genomic DNA of H. glycines in Southern blots. Full-length cDNAs of 21 clones were obtained by screening a gland-cell long-distance polymerase chain reaction cDNA library. Deduced proteins of 10 clones were preceded by a signal peptide for secretion, and PSORT II computer analysis predicted eight proteins as extracellular, one as nuclear, and one as plasmalemma localized. In situ hybridization showed that four of the predicted extracellular clones were expressed specifically in the dorsal gland cell, one in the subventral gland cells, and three in the intestine in H. glycines. The predicted nuclear clone and the plasmalemma-localized clone were expressed in the subventral gland cells and the dorsal gland cell, respectively. SSH is an efficient method for cloning putative parasitism genes encoding esophageal gland cell secretory proteins that may have a role in H. glycines parasitism of soybean.
Copyright Owner
The American Phytopathological Society
Copyright Date
2001
Language
en
File Format
application/pdf
Recommended Citation
Gao, Bingli; Allen, R.; Maier, Tom; Davis, Eric L.; Baum, Thomas J.; and Hussey, Richard S., "Identification of Putative Parasitism Genes Expressed in the Esophageal Gland Cells of the Soybean Cyst Nematode Heterodera glycines" (2001). Plant Pathology and Microbiology Publications. 161.
https://lib.dr.iastate.edu/plantpath_pubs/161
Included in
Agricultural Science Commons, Agriculture Commons, Bioinformatics Commons, Genetics and Genomics Commons, Plant Pathology Commons
Comments
This article is published as Gao, Bingli, R. Allen, Tom Maier, Eric L. Davis, Thomas J. Baum, and Richard S. Hussey. "Identification of putative parasitism genes expressed in the esophageal gland cells of the soybean cyst nematode Heterodera glycines." Molecular Plant-Microbe Interactions 14, no. 10 (2001): 1247-1254, doi: 10.1094/MPMI.2001.14.10.1247. Posted with permission.