Campus Units
Plant Pathology and Microbiology, Genetics
Document Type
Article
Publication Version
Published Version
Publication Date
2-2007
Journal or Book Title
Molecular Plant-Microbe Interactions
Volume
20
Issue
2
First Page
107
Last Page
119
DOI
10.1094/MPMI-20-2-0107
Abstract
Ethylene-responsive element-binding proteins (EREBPs) are plant-specific transcription factors, many of which have been linked to plant defense responses. Conserved EREBP domains bind to the GCC box, a promoter element found in pathogenesis-related (PR) genes. We previously identified an EREBP gene from soybean (GmEREBP1) whose transcript abundance decreased in soybean cyst-nematode-infected roots of a susceptible cultivar, whereas it increased in abundance in infected roots of a resistant cultivar. Here, we report further characterization of this gene. Transient expression analyses showed that GmEREBP1 is localized to the plant nucleus and functions as a transcriptional activator in soybean leaves. Transgenic soybean plants expressing GmEREBP1 activated the expression of the ethylene (ET)-responsive gene PR2 and the ET- and jasmonic acid (JA)-responsive gene PR3, and the salicylic acid (SA)-responsive gene PR1 but not the SA-responsive PR5. Similarly, transgenic Arabidopsis plants expressing GmEREBP1 showed elevated mRNA abundance of the ET-regulated gene PR3 and the ET- and JA-regulated defense-related gene PDF1.2 but not the ET-regulated GST2, and the SA-regulated gene PR1 but not the SA-regulated PR2 and PR5. Transgenic soybean and Arabidopsis plants inoculated with cyst nematodes did not display a significantly altered susceptibility to nematode infection. These results collectively show that GmEREBP1 functions as a transacting inducer of defense gene expression in both soybean and Arabidopsis and mediates the expression of both ET- and JA- and SA-regulated defense-related genes in these plant species.
Copyright Owner
The American Phytopathological Society
Copyright Date
2007
Language
en
File Format
application/pdf
Recommended Citation
Mazarei, Mitra; Elling, Axel A.; Maier, Tom R.; Puthoff, David P.; and Baum, Thomas J., "GmEREBP1 Is a Transcription Factor Activating Defense Genes in Soybean and Arabidopsis" (2007). Plant Pathology and Microbiology Publications. 166.
https://lib.dr.iastate.edu/plantpath_pubs/166
Included in
Agricultural Science Commons, Agriculture Commons, Agronomy and Crop Sciences Commons, Plant Breeding and Genetics Commons, Plant Pathology Commons
Comments
This article is published as Mazarei, Mitra, Axel A. Elling, Tom R. Maier, David P. Puthoff, and Thomas J. Baum. "GmEREBP1 is a transcription factor activating defense genes in soybean and Arabidopsis." Molecular Plant-Microbe Interactions 20, no. 2 (2007): 107-119, doi: 10.1094/MPMI-20-2-0107. Posted with permission.