Campus Units
Plant Pathology and Microbiology, Statistics
Document Type
Article
Publication Version
Published Version
Publication Date
3-2007
Journal or Book Title
Molecular Plant-Microbe Interactions
Volume
20
Issue
3
First Page
293
Last Page
305
DOI
10.1094/MPMI-20-3-0293
Abstract
Global analysis of gene expression changes in soybean (Glycine max) and Heterodera glycines (soybean cyst nematode [SCN]) during the course of infection in a compatible interaction was performed using the Affymetrix GeneChip soybean genome array. Among 35,611 soybean transcripts monitored, we identified 429 genes that showed statistically significant differential expression between uninfected and nematode-infected root tissues. These included genes encoding enzymes involved in primary metabolism; biosynthesis of phenolic compounds, lignin, and flavonoids; genes related to stress and defense responses; cell wall modification; cellular signaling; and transcriptional regulation. Among 7,431 SCN transcripts monitored, 1,850 genes showed statistically significant differential expression across different stages of nematode parasitism and development. Differentially expressed SCN genes were grouped into nine different clusters based on their expression profiles during parasitism of soybean roots. The patterns of gene expression we observed in SCN suggest coordinated regulation of genes involved in parasitism. Quantitative real-time reverse-transcription polymerase chain reaction confirmed the results of our microarray analysis. The simultaneous genome-wide analysis of gene expression changes in the host and pathogen during a compatible interaction provides new insights into soybean responses to nematode infection and the first profile of transcript abundance changes occurring in the nematode as it infects and establishes a permanent feeding site within a host plant root.
Copyright Owner
The American Phytopathological Society
Copyright Date
2007
Language
en
File Format
application/pdf
Recommended Citation
Ithal, Nagabhushana; Recknor, Justin; Nettleton, Dan; Hearne, Leonard; Maier, Tom; Baum, Thomas J.; and Mitchum, Melissa G., "Parallel Genome-Wide Expression Profiling of Host and Pathogen During Soybean Cyst Nematode Infection of Soybean" (2007). Plant Pathology and Microbiology Publications. 167.
https://lib.dr.iastate.edu/plantpath_pubs/167
Included in
Agricultural Science Commons, Agriculture Commons, Genetics and Genomics Commons, Plant Pathology Commons, Statistical Methodology Commons
Comments
This article is published as Ithal, Nagabhushana, Justin Recknor, Dan Nettleton, Leonard Hearne, Tom Maier, Thomas J. Baum, and Melissa G. Mitchum. "Parallel genome-wide expression profiling of host and pathogen during soybean cyst nematode infection of soybean." Molecular Plant-Microbe Interactions 20, no. 3 (2007): 293-305, doi: 10.1094/MPMI-20-3-0293. Posted with permission.