Campus Units
Plant Pathology and Microbiology, Plant Biology
Document Type
Article
Publication Version
Published Version
Publication Date
10-2015
Journal or Book Title
Phytopathology
Volume
105
Issue
10
First Page
1362
Last Page
1372
DOI
10.1094/PHYTO-02-15-0049-R
Abstract
Heterodera glycines, the soybean cyst nematode, is the number one pathogen of soybean (Glycine max). This nematode infects soybean roots and forms an elaborate feeding site in the vascular cylinder. H. glycines produces an arsenal of effector proteins in the secretory esophageal gland cells. More than 60 H. glycines candidate effectors were identified in previous gland-cell-mining projects. However, it is likely that additional candidate effectors remained unidentified. With the goal of identifying remaining H. glycines candidate effectors, we constructed and sequenced a large gland cell cDNA library resulting in 11,814 expressed sequence tags. After bioinformatic filtering for candidate effectors using a number of criteria, in situ hybridizations were performed in H. glycines whole-mount specimens to identify candidate effectors whose mRNA exclusively accumulated in the esophageal gland cells, which is a hallmark of many nematode effectors. This approach resulted in the identification of 18 new H. glycines esophageal gland-cell-specific candidate effectors. Of these candidate effectors, 11 sequences were pioneers without similarities to known proteins while 7 sequences had similarities to functionally annotated proteins in databases. These putative homologies provided the bases for the development of hypotheses about potential functions in the parasitism process.
Copyright Owner
The American Phytopathological Society
Copyright Date
2015
Language
en
File Format
application/pdf
Recommended Citation
Noon, Jason B.; Hewezi, Tarek; Maier, Thomas R.; Simmons, Carl; Wei, Jun-Zhi; Wu, Gusui; Llaca, Victor; Deschamps, Stéphane; Davis, Eric L.; Mitchum, Melissa G.; Hussey, Richard S.; and Baum, Thomas J., "Eighteen New Candidate Effectors of the Phytonematode Heterodera glycines Produced Specifically in the Secretory Esophageal Gland Cells During Parasitism" (2015). Plant Pathology and Microbiology Publications. 172.
https://lib.dr.iastate.edu/plantpath_pubs/172
Included in
Agricultural Science Commons, Agriculture Commons, Bioinformatics Commons, Genetics and Genomics Commons, Plant Pathology Commons
Comments
This article is published as Noon, J. B., Hewezi, T.,Maier, T. R., Simmons, C.,Wei, J., Wu, G., Llaca, V., Deschamps, S., Davis, E. L., Mitchum, M. G., Hussey, R. S., and Baum, T. J. 2015. Eighteen new candidate effectors of the phytonematode Heterodera glycines produced specifically in the secretory esophageal gland cells during parasitism. Phytopathology 105:1362-1372, doi: 10.1094/PHYTO-02-15-0049-R. Posted with permission.