Campus Units

Plant Pathology and Microbiology

Document Type

Article

Publication Version

Published Version

Publication Date

3-2002

Journal or Book Title

Journal of Nematology

Volume

34

Issue

1

First Page

12

Last Page

15

Abstract

Secretory proteins encoded by parasitism genes expressed in the esophageal gland cells of plant-parasitic nematodes play key roles in nematode-plant interactions. A fourth ß-1,4-endoglucanase full-length cDNA (designated Hg-eng-4) was isolated from a Heterodera glycines esophageal gland-cell long-distance polymerase chain reaction cDNA library. The cDNA hybridized to genomic DNA of H. glycines in Southern blots. The Hg-eng-4 cDNA contained an open reading frame encoding 352 amino acids, with the first 18 amino acids being a putative secretion signal. Hg-ENG-4 contained a family 5 endoglucanase catalytic domain and a peptide linker of repeat amino acids, but no cellulose binding domain. In-situ hybridization analyses showed that transcripts of Hg-eng-4 accumulated specifically in the subventral gland cells of pre-parasitic and parasitic second-stage juveniles of H. glycines.

Comments

This article is published as Gao, B. "Identification of a New ß-1, 4-endoglucanase Gene Expressed in the Esophageal Subventral Gland Cells of Heterodera glycines." Journal of Nematology 34, no. 1: 12-15. Posted with permission.

Copyright Owner

The Society of Nematologists

Language

en

File Format

application/pdf

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