Campus Units
Plant Pathology and Microbiology, Genetics, Botany
Document Type
Article
Publication Version
Published Version
Publication Date
6-2000
Journal or Book Title
Journal of Nematology
Volume
32
Issue
2
First Page
166
Last Page
173
Abstract
Genetic approaches are a powerful means to elucidate plant-pathogen interactions. An in vitro screening protocol was developed to identify Arabidopsis thaliana mutants with altered susceptibility to Heterodera schachtii, the sugar beet cyst nematode. In an initial screen of approximately 5,200 ethyl methanesulfonate-generated mutant plants, two stable mutations were identified. Both mutant lines were backcrossed and were found to harbor single recessive mutant alleles. Mutant line 2-4-6 shows an approximately two-fold increase in sedentary and developing nematodes, while mutant line 10-5-2 exhibits a significant decrease in susceptibility that manifests itself only after nematodes become sedentary. Analyses of progeny from crosses of lines 2-4-6 and 10-5-2 indicated that the two mutations are not allelic. However, the mutant gene in line 2-4-6 was found to be allelic to the previously identified mutant rhd1 and was termed rhd1-4. The mutant gene in line 10-5-2 was called asc1 for altered susceptibility to cyst nematodes. Our results demonstrate the feasibility of a nematological mutant screen and strengthen A. thaliana and H. schachtii as a model pathosystem.
Copyright Owner
The Society of Nematologists
Copyright Date
2000
Language
en
File Format
application/pdf
Recommended Citation
Baum, T. J.; Wubben, M. J. E. II; Hardy, K. A.; Su, H.; and Rodermel, S. R., "A Screen for Arabidopsis thalianaMutants with Altered Susceptibility to Heterodera schachtii" (2000). Plant Pathology and Microbiology Publications. 187.
https://lib.dr.iastate.edu/plantpath_pubs/187
Included in
Agricultural Science Commons, Botany Commons, Genetics and Genomics Commons, Plant Breeding and Genetics Commons, Plant Pathology Commons
Comments
This article is published as Baum, T. J., M. J. E. Wubben II, K. A. Hardy, H. Su, and S. R. Rodermel. "A Screen for Arabidopsis thalianaMutants with Altered Susceptibility to Heterodera schachtii." Journal of Nematology 32, no. 2: 166-173. Posted with permission.