Campus Units
Plant Pathology and Microbiology
Document Type
Article
Publication Version
Published Version
Publication Date
2-2018
Journal or Book Title
Frontiers in Plant Science
Volume
9
First Page
110
DOI
10.3389/fpls.2018.00110
Abstract
Goss's wilt (GW) of maize is caused by the Gram-positive bacterium Clavibacter michiganensis subsp. nebraskensis (Cmn) and has spread in recent years throughout the Great Plains, posing a threat to production. The genetic basis of plant resistance is unknown. Here, a simple method for quantifying disease symptoms was developed and used to select cohorts of highly resistant and highly susceptible lines known as extreme phenotypes (XP). Copy number variation (CNV) analyses using whole genome sequences of bulked XP revealed 141 genes containing CNV between the two XP groups. The CNV genes include the previously identified common rust resistant locus rp1. Multiple Rp1 accessions with distinct rp1 haplotypes in an otherwise susceptible accession exhibited hypersensitive responses upon inoculation. GW provides an excellent system for the genetic dissection of diseases caused by closely related subspecies of C. michiganesis. Further work will facilitate breeding strategies to control GW and provide needed insight into the resistance mechanism of important related diseases such as bacterial canker of tomato and bacterial ring rot of potato.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Copyright Owner
The Authors
Copyright Date
2018
Language
en
File Format
application/pdf
Recommended Citation
Hu, Ying; Ren, Jie; Peng, Zhao; Umana, Arnoldo A.; Le, Ha; Danilova, Tatiana; Fu, Junjie; Wang, Haiyan; Robertson, Alison; Hulbert, Scot H.; White, Frank F.; and Liu, Sanzhen, "Analysis of Extreme Phenotype Bulk Copy Number Variation (XP-CNV) Identified the Association of rp1 with Resistance to Goss's Wilt of Maize" (2018). Plant Pathology and Microbiology Publications. 226.
https://lib.dr.iastate.edu/plantpath_pubs/226
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Breeding and Genetics Commons, Plant Pathology Commons
Comments
This article is published as Hu, Ying, Jie Ren, Zhao Peng, Arnoldo A. Umana, Ha Le, Tatiana Danilova, Junjie Fu et al. "Analysis of Extreme Phenotype Bulk Copy Number Variation (XP-CNV) Identified the Association of rp1 with Resistance to Goss's Wilt of Maize." Frontiers in plant science 9 (2018): 110. doi: 10.3389/fpls.2018.00110. Posted with permission.