Bean pod mottle virus (BPMV) negatively affects soybean yield and quality, yet quantitative information on effect of time of BPMV infection on soybean yield and quality has not been reported. The impact of time of BPMV infection on soybean yield, yield components, and grain quality components were quantified during the 2006 and 2007 soybean growing seasons in Iowa. Soybean quadrats (30 cm in length) were established within soybean plots (‘NB3001’) that consisted of six rows and were 7.5 m long. Quadrats were sampled 9 times during the 2006 growing season and 10 times during the 2007 growing season, beginning 25 days after planting in both years. Sap was extracted from leaflet samples from each quadrat and tested for the presence or absence of BPMV by enzyme-linked immunosorbent assay. The day of year (DOY) and quadrat position when BPMV was first detected within each plot were recorded and mapped. Soybean yield, number of pods per plant, number of seed per pod, and 100-seed weight for each quadrat were determined. The relationship between time (DOY) of BPMV infection and soybean yield, soybean yield components, and soybean grain quality were then quantified using linear regression. DOY of BPMV infection within quadrats explained 89.7 and 57.9% of the variation in soybean grain yield in 2006 and 2007, respectively. Soybean yield damage functions (slopes) were 15.2 and 8.1 kg/ha per day, respectively, indicating that, for each day that BPMV infection was delayed, soybean yield increased by 15.2 kg/ha in 2006 and 8.1 kg/ha in 2007. The number of pods per plant increased by 0.15 pods for each day that BPMV infection was delayed (R² = 72.8%) in 2006 but there was no relationship in 2007. The 100-seed weight had a significant linear relationship with the DOY when BPMV was first detected within quadrats in 2006 (slope = 0.013, R² = 86.3%) but not in 2007. The percentage of mottled seed in 2006 decreased by 1% for each day that BPMV infection was delayed in 2006 (R²= 87.4%). Both protein and oil content were affected by the DOY that BPMV was first detected within quadrats in 2006 but not in 2007. This study demonstrated that time of BPMV infection can negatively affect soybean yield, yield components, and grain quality components when BPMV disease risk is high.