Campus Units

Plant Pathology and Microbiology

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

7-2018

Journal or Book Title

New Phytologist

Volume

219

Issue

2

First Page

697

Last Page

713

DOI

10.1111/nph.15179

Abstract

• Cyst nematodes deliver effector proteins into host cells to manipulate cellular processes and establish a metabolically hyperactive feeding site. The novel 30D08 effector protein is produced in the dorsal gland of parasitic juveniles, but its function has remained unknown.

• We demonstrate that expression of 30D08 contributes to nematode parasitism, the protein is packaged into secretory granules, and is targeted to the plant nucleus where it interacts with SMU2 (homologue of suppressor of mec-8 and unc-52 2), an auxiliary spliceosomal protein.

• We show that SMU2 is expressed in feeding sites and a smu2 mutant is less susceptible to nematode infection. In Arabidopsis expressing 30D08 under the SMU2 promoter, several genes were found to be alternatively spliced and the most abundant functional classes represented among differentially expressed genes were involved in RNA processing, transcription and binding, as well as in development, hormone and secondary metabolism representing key cellular processes known to be important for feeding site formation.

• In conclusion, we demonstrated that the 30D08 effector is secreted from the nematode and targeted to the plant nucleus where its interaction with a host auxiliary spliceosomal protein may alter the pre-mRNA splicing and expression of a subset of genes important for feeding site formation.

Comments

This is the peer reviewed version of the following article: Verma, Anju, Chris Lee, Stephanie Morriss, Fiona Odu, Charlotte Kenning, Nancy Rizzo, William G. Spollen et al. "The novel cyst nematode effector protein 30D08 targets host nuclear functions to alter gene expression in feeding sites." New Phytologist 219, no. 2 (2018): 697-713, which has been published in final form at doi: 10.1111/nph.15179. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.

Copyright Owner

The Authors, New Phytologist, New Phytologist Trust

Language

en

File Format

application/pdf

Published Version

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