Campus Units

Plant Pathology and Microbiology

Document Type

Article

Publication Version

Published Version

Publication Date

8-2018

Journal or Book Title

Molecular Plant Pathology

Volume

19

Issue

8

First Page

1887

Last Page

1907

DOI

10.1111/mpp.12666

Abstract

Pratylenchus penetrans is one of the most important species of root lesion nematodes (RLNs) because of its detrimental and economic impact in a wide range of crops. Similar to other plant‐parasitic nematodes (PPNs), P. penetrans harbours a significant number of secreted proteins that play key roles during parasitism. Here, we combined spatially and temporally resolved next‐generation sequencing datasets of P. penetrans to select a list of candidate genes aimed at the identification of a panel of effector genes for this species. We determined the spatial expression of transcripts of 22 candidate effectors within the oesophageal glands of P. penetrans by in situ hybridization. These comprised homologues of known effectors of other PPNs with diverse putative functions, as well as novel pioneer effectors specific to RLNs. It is noteworthy that five of the pioneer effectors encode extremely proline‐rich proteins. We then combined in situ localization of effectors with available genomic data to identify a non‐coding motif enriched in promoter regions of a subset of P. penetrans effectors, and thus a putative hallmark of spatial expression. Expression profiling analyses of a subset of candidate effectors confirmed their expression during plant infection. Our current results provide the most comprehensive panel of effectors found for RLNs. Considering the damage caused by P. penetrans, this information provides valuable data to elucidate the mode of parasitism of this nematode and offers useful suggestions regarding the potential use of P. penetrans‐specific target effector genes to control this important pathogen.

Comments

This article is published as Vieira, Paulo, Thomas R. Maier, Sebastian Eves‐van den Akker, Dana K. Howe, Inga Zasada, Thomas J. Baum, Jonathan D. Eisenback, and Kathryn Kamo. "Identification of candidate effector genes of Pratylenchus penetrans." Molecular plant pathology 19, no. 8 (2018): 1887-1907. doi: 10.1111/mpp.12666.

Rights

Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.

Language

en

File Format

application/pdf

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