Campus Units
Plant Pathology and Microbiology
Document Type
Article
Publication Version
Published Version
Publication Date
2019
Journal or Book Title
Journal of Experimental Botany
Volume
70
Issue
5
First Page
1653
Last Page
1668
DOI
10.1093/jxb/erz022
Abstract
Heterodera glycines, the soybean cyst nematode, penetrates soybean roots and migrates to the vascular cylinder where it forms a feeding site called the syncytium. MiRNA396 (miR396) targets growth-regulating factor (GRF) genes, and the miR396–GRF1/3 module is a master regulator of syncytium development in model cyst nematode H. schachtii infection of Arabidopsis. Here, we investigated whether this regulatory system operates similarly in soybean roots and is likewise important for H. glycines infection. We found that a network involving nine MIR396 and 23 GRF genes is important for normal development of soybean roots and that GRF function is specified in the root apical meristem by miR396. All MIR396 genes are down-regulated in the syncytium during its formation phase while, specifically, 11 different GRF genes are up-regulated. The switch to the syncytium maintenance phase coincides with up-regulation of MIR396 and down-regulation of the 11 GRF genes specifically via post-transcriptional regulation by miR396. Furthermore, interference with the miR396–GRF6/8–13/15–17/19 regulatory network, through either overexpression or knockdown experiments, does not affect the number of H. glycines juveniles that enter the vascular cylinder to initiate syncytia, but specifically inhibits efficient H. glycines development to adult females. Therefore, homeostasis in the miR396–GRF6/8–13/15–17/19 regulatory network is essential for productive H. glycines infections.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Copyright Owner
The Authors
Copyright Date
2019
Language
en
File Format
application/pdf
Recommended Citation
Noon, Jason B.; Hewezi, Tarek; and Baum, Thomas J., "Homeostasis in the soybean miRNA396–GRF network is essential for productive soybean cyst nematode infections" (2019). Plant Pathology and Microbiology Publications. 278.
https://lib.dr.iastate.edu/plantpath_pubs/278
Included in
Agricultural Science Commons, Agriculture Commons, Plant Breeding and Genetics Commons, Plant Pathology Commons
Comments
This article is published as Noon, Jason B., Tarek Hewezi, and Thomas J. Baum. "Homeostasis in the soybean miRNA396–GRF network is essential for productive soybean cyst nematode infections." Journal of experimental botany 70, no. 5 (2019): 1653-1668. doi: 10.1093/jxb/erz022.