Campus Units
Plant Pathology and Microbiology
Document Type
Article
Publication Version
Accepted Manuscript
Publication Date
10-2018
Journal or Book Title
Molecular Plant Pathology
Volume
19
Issue
10
First Page
2263
Last Page
2276
DOI
10.1111/mpp.12697
Abstract
Cyst nematodes are plant pathogens that infect a wide range of economically important crops. One parasitic mechanism employed by cyst nematodes is the production and in planta delivery of effector proteins to modify plant cells and suppress defenses to favor parasitism. This study focused on GLAND4, an effector of Heterodera glycines and H. schachtii, the soybean and sugar beet cyst nematodes, respectively. We showed that GLAND4 is recognized by the plant cellular machinery and is transported to the plant nucleus, an organelle where little is known about plant nematode effector functions. We showed that GLAND4 has DNA-binding ability and repressed reporter gene expression in a plant transcriptional assay. One DNA-fragment that bound to GLAND4 was localized in an Arabidopsis chromosomal region associated with the promoters of two lipid transfer protein (LTP) genes. These LTPs have known defense functions and are downregulated in the nematode feeding site. When expressed in Arabidopsis, the presence of GLAND4 caused downregulation of the two LTP genes in question, which was associated also with increased susceptibility to the plant-pathogenic bacterium Pseudomonas syringae. Furthermore, overexpression of one of the LTP genes reduced plant susceptibility to H. schachtii and P. syringae, confirming that LTP repression likely suppresses plant defenses. This study made GLAND4 one of a small subset of characterized plant nematode nuclear effectors and identified GLAND4 as the first DNA-binding plantparasitic nematode effector.
Copyright Owner
BSPP and John Wiley & Sons Ltd
Copyright Date
2018
Language
en
File Format
application/pdf
Recommended Citation
Barnes, Stacey N.; Wram, Catherine L.; Mitchum, Melissa G.; and Baum, Thomas J., "The plant‐parasitic cyst nematode effector GLAND4 is a DNA‐binding protein" (2018). Plant Pathology and Microbiology Publications. 281.
https://lib.dr.iastate.edu/plantpath_pubs/281
Included in
Agricultural Science Commons, Cell and Developmental Biology Commons, Entomology Commons, Genetics Commons, Plant Pathology Commons
Comments
This is the peer reviewed version of the following article: Barnes, Stacey N., Catherine L. Wram, Melissa G. Mitchum, and Thomas J. Baum. "The plant‐parasitic cyst nematode effector GLAND4 is a DNA‐binding protein." Molecular plant pathology 19, no. 10 (2018): 2263-2276, which has been published in final form at doi: 10.1111/mpp.12697. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.