Document Type

Article

Publication Version

Published Version

Publication Date

2011

Journal or Book Title

Acta Crystallographica Section F: Structural Biology and Crystallization Communications

Volume

67

Issue

5

First Page

561

Last Page

564

DOI

10.1107/S1744309111007196

Abstract

Barley yellow dwarf virus (BYDV) RNA lacks a 5′ m7GTP cap, yet it is translated efficiently because it contains a 105-base BYDV-like cap-independent translation element (BTE) in the 3′ untranslated region (UTR). To understand how the BTE outcompetes the host mRNA for protein-synthesis machinery, its three-dimensional structure is being determined at high resolution. The purification using transcription from DNA containing 2′-O-methyl nucleotides and preliminary crystallographic analyses of the BTE RNA are presented here. After varying the BTE sequence and crystallization-condition optimization, crystals were obtained that diffracted to below 5 Å resolution, with a complete data set being collected to 6.9 Å resolution. This crystal form indexes with an Rmerge of 0.094 in the monoclinic space group C2, with unit-cell parameters a = 316.6, b = 54.2, c = 114.5 Å, α = γ = 90, β = 105.1°.

Comments

This article is from Acta Crystallographica Section F: Structural Biology and Crystallization Communications 67 (2011): 561, doi: 10.1107/S1744309111007196. Posted with permission.

Rights

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Copyright Owner

International Union of Crystallography

Language

en

File Format

application/pdf

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