Document Type
Article
Publication Version
Published Version
Publication Date
2011
Journal or Book Title
Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume
67
Issue
5
First Page
561
Last Page
564
DOI
10.1107/S1744309111007196
Abstract
Barley yellow dwarf virus (BYDV) RNA lacks a 5′ m7GTP cap, yet it is translated efficiently because it contains a 105-base BYDV-like cap-independent translation element (BTE) in the 3′ untranslated region (UTR). To understand how the BTE outcompetes the host mRNA for protein-synthesis machinery, its three-dimensional structure is being determined at high resolution. The purification using transcription from DNA containing 2′-O-methyl nucleotides and preliminary crystallographic analyses of the BTE RNA are presented here. After varying the BTE sequence and crystallization-condition optimization, crystals were obtained that diffracted to below 5 Å resolution, with a complete data set being collected to 6.9 Å resolution. This crystal form indexes with an Rmerge of 0.094 in the monoclinic space group C2, with unit-cell parameters a = 316.6, b = 54.2, c = 114.5 Å, α = γ = 90, β = 105.1°.
Rights
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Copyright Owner
International Union of Crystallography
Copyright Date
2011
Language
en
File Format
application/pdf
Recommended Citation
Kraft, Jelena Jeremic; Hoy, Julie A.; and Miller, W. Allen, "Crystallization and preliminary X-ray diffraction analysis of the barley yellow dwarf virus cap-independent translation element" (2011). Plant Pathology and Microbiology Publications. 34.
https://lib.dr.iastate.edu/plantpath_pubs/34
Included in
Agricultural Science Commons, Biochemistry, Biophysics, and Structural Biology Commons, Plant Pathology Commons
Comments
This article is from Acta Crystallographica Section F: Structural Biology and Crystallization Communications 67 (2011): 561, doi: 10.1107/S1744309111007196. Posted with permission.