Document Type
Article
Publication Version
Published Version
Publication Date
9-1996
Journal or Book Title
Journal of Virology
Volume
70
Issue
9
First Page
5884
Last Page
5892
Abstract
Many viruses use stop codon readthrough as a strategy to produce extended coat or replicase proteins. The stop codon of the barley yellow dwarf virus (PAV serotype) coat protein gene is read through at a low rate. This produces an extended polypeptide which becomes part of the virion. We have analyzed the cis-acting sequences in the barley yellow dwarf virus PAV genome required for this programmed readthrough in vitro in wheat germ extracts and reticulocyte lysates and in vivo in oat protoplasts. Two regions 3' to the stop codon were required. Deletion of sections containing the first 5 of the 16 CCN NNN repeats located 3' of the stop codon greatly reduced readthrough in vitro and in vivo. Surprisingly, readthrough also required a second, more distal element that is located 697 to 758 bases 3' of the stop codon within the readthrough open reading frame. This element also functioned in vivo in oat protoplasts when placed more than 2 kb from the coat protein gene stop in the untranslated region following a GUS reporter gene. This is the first report of a long-range readthrough signal in viruses.
Copyright Owner
American Society for Microbiology
Copyright Date
1996
Language
en
File Format
application/pdf
Recommended Citation
Brown, Chris M.; Dinesh-Kumar, S. P.; and Miller, W. Allen, "Local and Distant Sequences Are Required for Efficient Readthrough of the Barley Yellow Dwarf Virus PAV Coat Protein Gene Stop Codon" (1996). Plant Pathology and Microbiology Publications. 50.
https://lib.dr.iastate.edu/plantpath_pubs/50
Included in
Agricultural Science Commons, Cell and Developmental Biology Commons, Molecular Biology Commons, Plant Pathology Commons
Comments
This article is from Journal of Virology 70 (1996): 5884. Posted with permission.