Document Type
Article
Publication Version
Published Version
Publication Date
6-2014
Journal or Book Title
Journal of Agriculture and Food Chemistry
Volume
62
Issue
27
First Page
6463
Last Page
6472
DOI
10.1021/jf5011897
Abstract
Field trials were conducted from 2007 to 2010 to compare grain fumonisin levels among non-Bt maize hybrids and Bt hybrids with transgenic protection against manual infestations of European corn borer (ECB) and Western bean cutworm (WBC). HPLC and ELISA were used to measure fumonisin levels. Results of the methods were highly correlated, but ELISA estimates were higher. Bt hybrids experienced less insect injury, Fusarium ear rot, and fumonisin contamination compared to non-Bt hybrids. WBC infestation increased fumonisin content compared to natural infestation in non-Bt and hybrids expressing Cry1Ab protein in five of eight possible comparisons; in Cry1F hybrids, WBC did not impact fumonisins. These results indicate that WBC is capable of increasing fumonisin levels in maize. Under WBC infestation, Cry1F mitigated this risk more consistently than Cry1Ab or non-Bt hybrids. Transgenically expressed Bt proteins active against multiple lepidopteran pests can provide broad, consistent reductions in the risk of fumonisin contamination.
Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Recommended Citation
Bowers, Erin Louise; Hellmich, Richard L.; and Munkvold, Gary P., "Comparison of Fumonisin Contamination Using HPLC and ELISA Methods in Bt and Near-Isogenic Maize Hybrids Infested with European Corn Borer or Western Bean Cutworm" (2014). Plant Pathology and Microbiology Publications. 52.
https://lib.dr.iastate.edu/plantpath_pubs/52
Comments
This article is from Journal of Agricultural and Food Chemistry 62 (2014): 6463, doi:10.1021/jf5011897.