Title
Differential accumulation of host mRNAs on polyribosomes during obligate pathogen-plant interactions
Campus Units
Plant Pathology and Microbiology, Statistics, Plant Biology, Bioinformatics and Computational Biology
Document Type
Article
Publication Version
Published Version
Publication Date
2012
Journal or Book Title
Molecular BioSystems
Volume
8
First Page
2153
Last Page
2165
DOI
10.1039/C2MB25014D
Abstract
Plant pathogens elicit dramatic changes in the expression of host genes during both compatible and incompatible interactions. Gene expression profiling studies of plant-pathogen interactions have only considered messenger RNAs (mRNAs) present in total RNA, which contains subpopulations of actively translated mRNAs associated with polyribosomes (polysomes) and non-translated mRNAs that are not associated with polysomes. The goal of this study was to enhance previous gene expression analyses by identifying host mRNAs that become differentially associated with polysomes following pathogen inoculation. Total and polysomal RNA were extracted from barley (Hordeum vulgare) plants at 32 h after inoculation withBlumeria graminis f. sp. hordei, and Arabidopsis thaliana plants at 10 days after inoculation withTurnip mosaic virus. Gene expression profiles were obtained for each pathosystem, which represent diverse plant host-obligate pathogen interactions. Using this approach, host mRNAs were identified that were differentially associated with polysomes in response to pathogen treatment. Approximately 18% and 26% of mRNAs represented by probe sets on the Affymetrix Barley1 and Arabidopsis ATH1 GeneChips, respectively, differentially accumulated in the two populations in one or more combinations of treatment and genotype. Gene ontology analysis of mRNAs sharing the same pattern of accumulation in total and polysomal RNA identified gene sets that contained a significant number of functionally related annotations, suggesting both transcript accumulation and recruitment to polyribosomes are coordinately regulated in these systems.
Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Recommended Citation
Moeller, Jackson R.; Moscou, Matthew J.; Bancroft, Timothy J.; Skadsen, Ronald W.; Wise, Roger P.; and Whitham, Steven A., "Differential accumulation of host mRNAs on polyribosomes during obligate pathogen-plant interactions" (2012). Plant Pathology and Microbiology Publications. 68.
https://lib.dr.iastate.edu/plantpath_pubs/68
Included in
Agricultural Science Commons, Agriculture Commons, Bioinformatics Commons, Biostatistics Commons, Computational Biology Commons, Plant Biology Commons, Plant Pathology Commons
Comments
This article is from Molcular BioSystems 8 (2012): 2153–2165, doi:10.1039/C2MB25014D.