Campus Units
Plant Pathology and Microbiology, Bioinformatics and Computational Biology
Document Type
Article
Publication Version
Published Version
Publication Date
1-2009
Journal or Book Title
Plant Physiology
Volume
149
Issue
1
First Page
271
Last Page
285
DOI
10.1104/pp.108.129031
Abstract
Plants have evolved complex regulatory mechanisms to control the defense response against microbial attack. Both temporal and spatial gene expression are tightly regulated in response to pathogen ingress, modulating both positive and negative control of defense. BLUFENSIN1 (BLN1), a small peptide belonging to a novel family of proteins in barley (Hordeum vulgare), is highly induced by attack from the obligate biotrophic fungus Blumeria graminis f. sp. hordei (Bgh), casual agent of powdery mildew disease. Computational interrogation of the Bln1 gene family determined that members reside solely in the BEP clade of the Poaceae family, specifically, barley, rice (Oryza sativa), and wheat (Triticum aestivum). Barley stripe mosaic virus-induced gene silencing of Bln1 enhanced plant resistance in compatible interactions, regardless of the presence or absence of functional Mla coiled-coil, nucleotide-binding site, Leu-rich repeat alleles, indicating that BLN1 can function in an R-gene-independent manner. Likewise, transient overexpression of Bln1 significantly increased accessibility toward virulent Bgh. Moreover, silencing in plants harboring the Mlo susceptibility factor decreased accessibility to Bgh, suggesting that BLN1 functions in parallel with or upstream of MLO to modulate penetration resistance. Collectively, these data suggest that the grass-specific Bln1 negatively impacts basal defense against Bgh.
Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Recommended Citation
Meng, Yan; Moscou, Matthew J.; and Wise, Roger P., "Blufensin1 Negatively Impacts Basal Defense in Response to Barley Powdery Mildew" (2009). Plant Pathology and Microbiology Publications. 70.
https://lib.dr.iastate.edu/plantpath_pubs/70
Included in
Agricultural Science Commons, Agriculture Commons, Bioinformatics Commons, Plant Pathology Commons
Comments
This article is from Plant Physiology 149 (2009): 271–285, doi:10.1104/pp.108.129031.