Plant Pathology and Microbiology
Journal or Book Title
BMC Research Notes
Bean pod mottle virus (BPMV) based virus-induced gene silencing (VIGS) vectors have been developed and used in soybean for the functional analysis of genes involved in disease resistance to foliar pathogens. However, BPMV-VIGS protocols for studying genes involved in disease resistance or symbiotic associations with root microbes have not been developed.
Here we describe a BPMV-VIGS protocol suitable for reverse genetic studies in soybean roots. We use this method for analyzing soybean genes involved in resistance to soybean cyst nematode (SCN). A detailed SCN screening pipeline is described.
The VIGS method described here provides a new tool to identify genes involved in soybean-nematode interactions. This method could be adapted to study genes associated with any root pathogenic or symbiotic associations.
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Kandoth et al.
Kandoth, Pramod K.; Heinz, Robert; Yeckel, Gregory; Gross, Nathan W.; Juvale, Parijat S.; Hill, John H.; Whitham, Steven A.; Baum, Thomas J.; and Mitchum, Melissa G., "A virus-induced gene silencing method to study soybean cyst nematode parasitism in Glycine max" (2013). Plant Pathology and Microbiology Publications. 85.