Campus Units
Plant Pathology and Microbiology
Document Type
Article
Publication Version
Published Version
Publication Date
2011
Journal or Book Title
Biotechnology Letters
Volume
33
First Page
2217
Last Page
2223
DOI
10.1007/s10529-011-0682-9
Abstract
Analysis of an organism’s genetic diversity requires a method that gives reliable, reproducible results. Microsatellites are robust markers, however, detection of allele sizes can be difficult with some systems as well as consistency among laboratories. In this study, our two laboratories used 219 isolates of Phytophthora sojae to compare three microsatellite methods. Two capillary electrophoresis methods, the Applied Biosystems 3730 Genetic Analyzer and the CEQ 8000 Genetic Analysis system, detected an average of 2.4-fold more alleles compared to gel electrophoresis with a mean of 8.8 and 3.6 alleles per locus using capillary and gel methods, respectively. The two capillary methods were comparable, although allele sizes differed consistently by an average of 3.2 bp across isolates. Differences between capillary methods could be overcome if reference standard DNA genotypes are shared between collaborating laboratories.
Rights
This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
Copyright Owner
The Authors
Copyright Date
2011
Language
en
File Format
application/pdf
Recommended Citation
Stewart, Silvina Maria; Wickramasinghe, Damitha; Dorrance, Anne E.; and Robertson, Alison E., "Comparison of three microsatellite analysis methods for detecting genetic diversity in Phytophthora sojae (Stramenopila: Oomycete)" (2011). Plant Pathology and Microbiology Publications. 89.
https://lib.dr.iastate.edu/plantpath_pubs/89
Comments
This is an article from Biotechnology Letters 33 (2011): 2217, doi:10.1007/s10529-011-0682-9. Posted with permission.