Campus Units
Plant Pathology and Microbiology
Document Type
Article
Publication Version
Published Version
Publication Date
6-2011
Journal or Book Title
Journal of Bacteriology
Volume
193
Issue
12
First Page
3033
Last Page
3041
DOI
10.1128/JB.00160-11
Abstract
Pseudomonas aeruginosa uses the quaternary amine choline as a carbon source, osmoprotectant, and macromolecular precursor. The importance of choline in P. aeruginosa physiology is highlighted by the presence of multiple known and putative choline transporters encoded within its genome. This report describes the relative roles of three choline transporters, the ABC transporter CbcXWV and two symporters, BetT1 and BetT3, in P. aeruginosa growth on choline under osmotic conditions that are physiologically relevant to eukaryotic hosts. The increased lag phases exhibited by the ΔbetT1 and ΔbetT1 ΔbetT3 mutants relative to the wild type upon transfer to medium with choline as a sole carbon source suggested roles for BetT1 and BetT3 in cells newly exposed to choline. BetT3 and CbcXWV, but not BetT1, were sufficient to support growth on choline. betT1 and betT3expression was regulated by the repressor BetI and choline, whereas cbcXWVexpression was induced by the activator GbdR and glycine betaine. The data support a model in which, upon transfer to a choline-based medium, the glycine betaine derived from choline taken up by BetT1 and BetT3 promotes subsequent GbdR-mediated cbcXWV induction. Furthermore, growth data indicated that the relative contributions of each transporter varied under different conditions, as BetT1 and CbcXWV were the primary choline transporters under hypo-osmolar conditions whereas BetT3 was the major choline transporter under hyperosmolar conditions. This work represents the first systematic approach to unravel the mechanisms of choline uptake in P. aeruginosa, which has the most complex bacterial choline uptake systems characterized to date.
Copyright Owner
American Society for Microbiology
Copyright Date
2011
Language
en
File Format
application/pdf
Recommended Citation
Malek, Adel A.; Chen, Chiliang; Wargo, Matthew J.; Beattie, Gwyn A.; and Hogan, Deborah A., "Roles of Three Transporters, CbcXWV, BetT1, and BetT3, in Pseudomonas aeruginosa Choline Uptake for Catabolism" (2011). Plant Pathology and Microbiology Publications. 98.
https://lib.dr.iastate.edu/plantpath_pubs/98
Included in
Agricultural Science Commons, Agriculture Commons, Plant Biology Commons, Plant Breeding and Genetics Commons, Plant Pathology Commons
Comments
This article is from Journal of Bacteriology 193 (2011): 3033, doi: 10.1128/JB.00160-11. Posted with permission.