Degree Type

Dissertation

Date of Award

1995

Degree Name

Doctor of Philosophy

Department

Genetics, Development and Cell Biology

First Advisor

Jorgen Johansen

Second Advisor

Kristen Johansen

Abstract

The lan 3-6 monoclonal antibody recognizes a subpopulation of peripheral neurons whose afferents segregate into a single fascicle within the developing leech central nervous system. The lan 3-6 monoclonal antibody was used to isolate a clone encoding a novel protein, calsensin, which contains two EF-hand calcium-binding domains. In situ hybridizations with calsensin riboprobes, Western blots of lan 3-6 monoclonal antibody immunoprecipitations, and immunohistochemistry with polyclonal antibodies generated against a partially-purified calsensin fusion protein confirmed that the sequence encoding the lan 3-6 antigen has been identified. Partially-purified calsensin fusion protein binds [superscript]45Ca[superscript]2+ in vitro, suggesting that the calsensin calcium-binding domains are likely to function in vivo. Immunoaffinity purification with lan 3-6 antibodies resulted in the copurification of a 200,000 M[subscript] r protein with calsensin in two different leech species. Thus the molecular features and interactions of calsensin and its limited distribution to axons forming a single fascicle are consistent with the hypothesis that it may mediate calcium-dependent signal transduction events in the growth cones and axons leading to specific pathway selection.

DOI

https://doi.org/10.31274/rtd-180813-11072

Publisher

Digital Repository @ Iowa State University, http://lib.dr.iastate.edu/

Copyright Owner

Kristen Kay Briggs

Language

en

Proquest ID

AAI9531721

File Format

application/pdf

File Size

68 pages

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