Date of Award
Doctor of Philosophy
Zoology and Genetics
Alternative DNA conformations have been known to exist since before the elucidation of the double helix. The study of alternative DNA conformations formed by telomeric sequences has led to detailed studies of biologically relevant DNA capable of forming unusual structures. In order to understand the biological role of alternative DNA conformations formed by telomeric DNA, proteins which bind these structures have been identified and characterized;The initial portion of my research involved the identification of a protein from Tetrahymena thermophila which binds preferentially to parallel-stranded G-quartet structures. Competition and binding assays demonstrated that the protein, TGP (Tetrahymena G4 binding protein), binds to parallel G-DNA structures but not to antiparallel G-DNA structures or to non-G-DNA duplexes and single stranded oligonucleotides;This initial identification and characterization was followed up by attempts to purify TGP. Cation exchange and Y4-affinity chromatography partially purified two proteins (83 and 50 kDa) that coelute with peak TGP activity. UV cross-linking analysis confirmed the involvement of the 83 kDa protein as a main component of the TGP/G4-DNA complex.
Digital Repository @ Iowa State University, http://lib.dr.iastate.edu/
Ted Patrick Schierer
Schierer, Ted Patrick, "Identification and characterization of G4-DNA binding proteins from Tetrahymena thermophila " (1995). Retrospective Theses and Dissertations. 10979.