Date of Award
Doctor of Philosophy
Randy C. Shoemaker
The objectives of the studies were to map genes affecting iron deficiency chlorosis in soybean, to test the hypothesis that two genetic mechanisms control iron deficiency chlorosis in soybean, and to determine the effectiveness of nutrient solution evaluation for iron deficiency chlorosis in soybean. Chlorosis symptoms of one hundred and twenty F2:4 lines from a Pride B216 x A15 population, and 92 F2:4 lines from an Anoka x A7 population grown in a field of calcareous soil in 1993 and 1994 were evaluated by visual scores and spectrometric chlorophyll determinations. Each population also was evaluated separately with two cycles of nutrient solution tests in the greenhouse. Eighty-nine RFLP and ten SSR markers in the Pride B216 x A15 population, and 82 RFLP, 14 SSR and I (hilum color) markers in the Anoka x A7 population were used to construct linkage maps and to locate quantitative trait loci (QTL);In the field studies, one major gene and two QTL in the Anoka x A7 population, and seven QTL in the Pride B216 x A15 population were mapped. The results demonstrated the validity of the previous hypothesis of two separate genetic mechanisms controlling iron deficiency chlorosis in soybean;Because most QTL detected in field tests also were detected in the nutrient solution tests, these results verified that similar genetic mechanisms control iron deficiency chlorosis of soybean in field and in nutrient solution conditions. The results confirmed that nutrient solution tests are effective predictors of iron deficiency chlorosis response in field-grown soybean.
Digital Repository @ Iowa State University, http://lib.dr.iastate.edu/
Lin, Shun-Fu, "Molecular marker analysis of iron efficiency in soybean (Glycine max (L.) Merr.) " (1996). Retrospective Theses and Dissertations. 11162.