Degree Type
Dissertation
Date of Award
1997
Degree Name
Doctor of Philosophy
Department
Psychology
Major
Neuroscience
First Advisor
Richard A. Hughes
Abstract
Experiment 1. Chick embryos received a single DFP exposure (0, 50, 100, 200, 400, 800, 1600, 3200 [mu]g/kg) on embryogenesis day (E) 11. DFP was fatal to all embryos at 400 [mu]g/kg and above, but did not affect hatch success at lower doses. Prenatal DFP reduced hatch weight and increased weight by posthatch day (PHD) 14. Prenatal DFP produced sex- and dose-dependent changes in distress vocalizations (DVs), but not activity on PHD 1. DFP increased tonic immobility duration (TI) on PHD 14. DFP increased lipopolysaccharide-induced lymphocyte proliferation in blood harvested on PHD 16 in females;Experiment 2. Daily DFP (0, 50, 100, or 200 [mu]g/kg) on E 10-12 reduced hatch success. DFP reduced hatch weights in males, but not females. Prenatal DFP, 200 [mu]g/kg, increased weight by PHD 14. DFP did not affect DVs, but increased activity in males at 50 [mu]g/kg and females at 100 [mu]g/kg on PHD 1. DFP increased TI in females, but not males, on PHD 14. DFP decreased pokeweed mitogen-induced and increased lipopolysaccaride-induced lymphocyte proliferation in blood and thymus (respectively) harvested on PHD 16;Experiment 3. Daily DFP (0, 100, or 200 [mu]g/kg) on E 10-12 reduced hatch success, but did not affect weight at hatch or PHD 14. Chicks received 0.0, 0.5, or 1.5 mg/kg scopolamine 30 min before behavioral tests. Prenatal DFP (100 [mu]g/kg) decreased DVs and 1.5 mg/kg scopolamine reversed the effect on PHD 1; there were no activity effects. Scopolamine, 0.5 mg/kg reduced TI except at 200 [mu]g/kg DFP on PHD 14;Experiment 4. Daily DFP (0, 150, or 200 [mu]g/kg) on E 10-12 did not affect hatch success or hatch weight, but increased weight by PHD 14. Chicks received 0.0, 0.25, or 0.5 mg/kg physostigmine 30 min before behavioral tests. On PHD 1, 0.25 physostigmine increased DVs in chicks at 150 [mu]g/kg DFP; activity was not affected. DFP increased TI in females, but not males, and PHY increased TI in females at 0 [mu]g/kg DFP on PHD 18;These data demonstrate perinatal consequences of prenatal DFP that endure into the third week posthatch and are sensitive to cholinergic manipulation.
DOI
https://doi.org/10.31274/rtd-180813-17309
Publisher
Digital Repository @ Iowa State University, http://lib.dr.iastate.edu/
Copyright Owner
Michael Raymond Baker
Copyright Date
1997
Language
en
Proquest ID
AAI9737684
File Format
application/pdf
File Size
84 pages
Recommended Citation
Baker, Michael Raymond, "Perinatal behavioral and immunological effects of prenatal diisopropyl fluorophosphate exposure in domestic fowl " (1997). Retrospective Theses and Dissertations. 11770.
https://lib.dr.iastate.edu/rtd/11770
Included in
Animal Sciences Commons, Biological Psychology Commons, Medical Pharmacology Commons, Medical Toxicology Commons, Neuroscience and Neurobiology Commons, Pharmacology Commons, Physiology Commons, Psychiatry and Psychology Commons, Toxicology Commons, Veterinary Physiology Commons
