Degree Type

Dissertation

Date of Award

2000

Degree Name

Doctor of Philosophy

Department

Chemistry

First Advisor

Edward S. Yeung

Abstract

The purpose of this research is to develop a UV-vis absorbance detection approach for multiplexed capillary electrophoresis, and explore the use of multiplexed capillary electrophoresis with UV-vis absorbance detection as a high-throughput analysis technique for various types of applications;We first introduced a novel absorbance detection approach for 96-capillary array electrophoresis. The approach involved the use of a 1024-pixel linear photodiode array on which a 96-capillary array was imaged by a camera lens. The detection limit for Rhodamine 6G for each capillary in the multiplexed array was about 1.8 x 10--8 M (S/N = 2). The cross talk between adjacent capillaries is less than 0.2%. Uniform separation efficiency and signal to noise ratio were obtain across the capillary array. Simultaneous analysis of 96 samples using capillary zone electrophoresis (CZE) was demonstrated;We further successfully applied the 96-capillary array electrophoresis system with UV-vis absorbance detection for high-throughput HIV diagnosis, genetic typing, enzyme assay, protein analysis by peptide mapping, and combinatorial screening of homogeneous catalytic reactions.

DOI

https://doi.org/10.31274/rtd-180813-13592

Publisher

Digital Repository @ Iowa State University, http://lib.dr.iastate.edu/

Copyright Owner

Xiaoyi Gong

Language

en

Proquest ID

AAI9990446

File Format

application/pdf

File Size

143 pages

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