Degree Type
Thesis
Date of Award
2006
Degree Name
Master of Science
Department
Food Science and Human Nutrition
First Advisor
Tong Wang
Abstract
Sphingolipids (SL) are both structural and functional lipids with potential health benefits. To determine the relationship between palmitate and SL contents, methods for column separation and quantification using high-performance liquid chromatography - evaporative light scattering detector were developed for glucosylceramide (GlcCer) and ceramide (Cer) in 15 soybeans lines in which palmitate content ranged from 3.7 to 40.3%. Significant differences were observed among lines for GlcCer (83.4-397.6 nmol/g) and Cer (8.4-20.7 nmol/g) contents on a dry weight basis (dwb). But these contents were not significantly affected by the palmitate content of soybeans seeds. To evaluate the SL and phospholipid (PL) contents during seed development, soybean seeds of three cultivars were harvested at 5-day intervals from 28 to 68 days after flowering. Averaged across cultivars, the contents (dwb) decreased from 51.4 to 22.2 nmol/g for Cer, from 522.8 to 135.8 nmol/g for GlcCer, and from 9.1 to 3.5% for PL percentage relative to the total lipid.
DOI
https://doi.org/10.31274/rtd-180813-8738
Publisher
Digital Repository @ Iowa State University, http://lib.dr.iastate.edu
Copyright Owner
Liping Wang
Copyright Date
2006
Language
en
Proquest ID
AAI1439834
File Format
application/pdf
File Size
68 pages
Recommended Citation
Wang, Liping, "Soybean sphingolipid content as affected by palmitate content and by seed development stage " (2006). Retrospective Theses and Dissertations. 1371.
https://lib.dr.iastate.edu/rtd/1371
Included in
Agriculture Commons, Analytical Chemistry Commons, Bioresource and Agricultural Engineering Commons
