Degree Type

Thesis

Date of Award

2007

Degree Name

Master of Science

Department

Theses & dissertations (Interdisciplinary)

Major

Plant Physiology

First Advisor

Kan Wang

Abstract

Signaling components such as mitogen-activated protein kinases (MAPKs) regulate stress-induced pathways and are potentially a powerful means of genetically engineering plant tolerance to abiotic stress. The kinase domain of tobacco mitogen-activated protein kinase Nicotiana protein kinase 1 (NPK1) into rice cultivar Nipponbare. Four-week old transgenic and wild type plants treated with moderate (150mM) or severe (300mM) salt stress did not differ in visible signs of leaf damage, electrolyte leakage, malondialdehyde content or ion content within leaves. Expression of caNPK1 alters gene transcript abundance in the absence of stress as assessed by microarray analysis. Genes potentially related to flowering and development are downregulated while biotin biosynthesis and methionine recycling pathway as well as thirteen transcripts with matches to stress-related genes appear upregulated in the transgenic plants. Quantitative real-time PCR analysis indicates no significant difference in expression of several known rice MAPKs between caNPK1 transgenics and wild type.

DOI

https://doi.org/10.31274/rtd-180813-15844

Publisher

Digital Repository @ Iowa State University, http://lib.dr.iastate.edu/

Copyright Owner

Andréa Lu Scarpa

Language

en

Proquest ID

AAI1447513

OCLC Number

224513945

ISBN

9780549334699

File Format

application/pdf

File Size

135 pages

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